Park Jin Hyoung, Kim Doyeon, Shin Byung Seop
1 Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea.
2 Department of Anesthesiology and Pain Medicine, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, Republic of Korea.
J Int Med Res. 2018 Nov;46(11):4705-4716. doi: 10.1177/0300060518795583. Epub 2018 Sep 9.
We compared the effects of two anesthetics, isoflurane and propofol, on the nuclear or cytosolic localization of nuclear factor erythroid 2-related factor 2 (Nrf2), mRNA expression levels of excitatory amino acid carrier 1 (EAAC1), and glutathione (GSH) protein levels in the rat hippocampus.
Fifty-two adult male Sprague-Dawley rats were randomly divided into three groups: a control group, a group that received propofol for 240 minutes (P240), and a group that received isoflurane for 240 minutes (I240). We compared GSH protein and EAAC1 mRNA expression levels in the rat hippocampus and evaluated Nrf2 content in cytosolic and nuclear fractions in the three groups.
GSH protein and EAAC1 mRNA expression levels were significantly higher in the I240 and P240 groups compared with the control group. The I240 and P240 groups showed lower Nrf2 protein levels in the cytosolic fractions, but higher levels in the nuclear fractions compared with the control group.
Treatment with isoflurane or propofol may enhance GSH production by facilitating translocation of Nrf2 into the nucleus and increasing EAAC1mRNA expression in the rat hippocampus. Isoflurane and propofol show similar profiles in EAAC1 expression-associated GSH production.
我们比较了两种麻醉剂异氟烷和丙泊酚对大鼠海马体中核因子红细胞2相关因子2（Nrf2）的核定位或胞质定位、兴奋性氨基酸转运体1（EAAC1）的mRNA表达水平以及谷胱甘肽（GSH）蛋白水平的影响。
52只成年雄性Sprague-Dawley大鼠随机分为三组：对照组、接受丙泊酚240分钟的组（P240）和接受异氟烷240分钟的组（I240）。我们比较了大鼠海马体中GSH蛋白和EAAC1 mRNA的表达水平，并评估了三组中胞质和核部分的Nrf2含量。
与对照组相比，I240组和P240组的GSH蛋白和EAAC1 mRNA表达水平显著更高。与对照组相比，I240组和P240组的胞质部分Nrf2蛋白水平较低，但核部分水平较高。
异氟烷或丙泊酚治疗可能通过促进Nrf2易位至细胞核并增加大鼠海马体中EAAC1 mRNA表达来增强GSH生成。异氟烷和丙泊酚在与EAAC1表达相关联GSH生成方面表现出相似特征。
