Khlusov I A, Litvinova L S, Shupletsova V V, Dunets N A, Khaziakhmatova O G, Yurova K A, Khlusova M Yu, Sharkeev Yu P
Tsitologiia. 2016;58(10):778-84.
Human leukemic T-lymphoblastoid cells (hereinafter Jurkat T-cells) were used to model T-lymphocytes morphofunctional reaction to 24-h in vitro contact with relief (roughness index Ra = 2.2--2.7 mm) pure titanium substrates (12_12_1 mm3) covered by calcium phosphate (CP) bilateral coating that was prepared by micro-arc method. Jurkat T-cell culture on plastic surface of well plate (2D control of culture growth), as well as the cells that contacted for 24 h with oxide (TiO2) micro-arc coating on pure titanium substrate (3D control) served as comparison tests. 27--98 % of immortalized cells in 2D control culture had CD3+CD4+CD71+CD45RA+ immunophenotype and secreted IL-2, IL-4, IL-8, IL-10 and TNFa, but not IL-1b and IL-6. Other markers of cell activation, differentiation, maturation and death (CD8, CD16, CD56, CD25, CD95) were found at 0—2.5% of the cell population. Microtextured CP surface elevated statistically IL-8 outcome to 183 and 160 % of corresponding control values in 2D- and 3D-cultures of Jurkat T-cells. CD4/CD8 ratio fell to 9 : 1 (at 13 : 1 and 82 : 1 in 2D- and 3D-controls, respectively) both by CD4+ depletion and by CD8+ cell percent raise. Total amount of cells (TAC) in Jurkat T-cell culture after 24-h contact with CP coating was decreased to 88 % 2D control level (P < 0.04) that could favor to a suppression of cell division. TAC reduction in Jurkat T-cell culture was accompanied by accelerated IL-8 spontaneous secretion (r = –0.97, P < 0.00009). For all this, IL-8 induced apoptosis (r = 0.94, P < 0.0001) in low concentrations (pg/ml). The obtained result is the feature of Jurkat T-cells reaction on CP but not TiO2 coatings, and it may be used in case of materials selection for endoprosthesis replacement and fracture osteosynthesis in patients suffering by hematological and bone malignancies.
人白血病T淋巴母细胞（以下简称Jurkat T细胞）被用于模拟T淋巴细胞与通过微弧法制备的双侧磷酸钙（CP）涂层覆盖的纯钛基底（12×12×1 mm³）体外接触24小时后的形态功能反应。粗糙度指数Ra = 2.2 - 2.7 mm。在孔板塑料表面培养Jurkat T细胞（培养生长的二维对照），以及与纯钛基底上的氧化物（TiO₂）微弧涂层接触24小时的细胞（三维对照）作为比较试验。在二维对照培养中，27% - 98%的永生化细胞具有CD3⁺CD4⁺CD71⁺CD45RA⁺免疫表型，并分泌IL - 2、IL - 4、IL - 8、IL - 10和TNFα，但不分泌IL - 1β和IL - 6。细胞活化、分化、成熟和死亡的其他标志物（CD8、CD16、CD56、CD25、CD95）在细胞群体中的比例为0 - 2.5%。微纹理CP表面在Jurkat T细胞的二维和三维培养中，使IL - 8的分泌量在统计学上升至相应对照值的183%和160%。CD4/CD8比值降至9 : 1（二维对照和三维对照中分别为13 : 1和82 : 1），这是由于CD4⁺细胞减少和CD8⁺细胞百分比增加所致。与CP涂层接触24小时后，Jurkat T细胞培养中的细胞总数（TAC）降至二维对照水平的88%（P < 0.04），这可能有利于抑制细胞分裂。Jurkat T细胞培养中TAC的减少伴随着IL - 8自发分泌的加速（r = - 0.97，P < 0.00009）。尽管如此，低浓度（pg/ml）的IL - 8可诱导细胞凋亡（r = 0.94，P < 0.0001）。所获得的结果是Jurkat T细胞对CP涂层而非TiO₂涂层反应的特征，它可用于为患有血液系统和骨恶性肿瘤的患者选择假体置换和骨折骨固定材料的情况。
