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问号钩端螺旋体波摩那血清型溶血素与鞘磷脂酶C的共纯化

Copurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C.

作者信息

Bernheimer A W, Bey R F

出版信息

Infect Immun. 1986 Oct;54(1):262-4. doi: 10.1128/iai.54.1.262-264.1986.

DOI:10.1128/iai.54.1.262-264.1986
PMID:3019890
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260148/
Abstract

The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase C is responsible for hemolysis.

摘要

当进行等电点分级分离时，问号钩端螺旋体波摩那血清型培养物上清液的溶血活性和鞘磷脂酶C活性倾向于共同纯化。两种活性主要聚焦在pH 8.1。结合其他间接证据考虑，结果得出鞘磷脂酶C导致溶血的结论。

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