Yanagihara Y, Kojima T, Mifuchi I
Microbiol Immunol. 1982;26(7):547-56.
Hemolytic activity of the culture supernatant of Leptospira interrogans serovar canicola strain Moulton grown in protein-free medium was demonstrated. The activity began to appear in the late logarithmic phase of growth of the organism and reached a plateau after 2 weeks of cultivation. It was inactivated by the addition of dipotassium ethylenediaminetetraacetate, but was effectively restored by Mg2+. Hemolysis by the culture supernatant was stimulated by "hot-cold" incubation. Sleep erythrocytes treated with the culture supernatant of the organism were transformed into spherocytes, in which invagination was observed. A hemolysis inhibitor in rabbit serum was found to be in the chloroform-methanol soluble fraction of the serum. The hemolysin of Leptospira may be phospholipase C.
证明了问号钩端螺旋体犬型菌株莫尔顿在无蛋白培养基中生长的培养上清液具有溶血活性。该活性在生物体生长的对数后期开始出现，并在培养2周后达到平稳期。加入乙二胺四乙酸二钾可使其失活，但Mg2+可有效恢复其活性。“热-冷”孵育可刺激培养上清液的溶血作用。用该生物体的培养上清液处理的静息红细胞转变为球形红细胞，观察到有内陷现象。发现兔血清中的溶血抑制剂存在于血清的氯仿-甲醇可溶部分。钩端螺旋体的溶血素可能是磷脂酶C。
