Kurosumi M, Ishimura K, Yoshinaga T, Fujita H, Tamaoki B
Histochemistry. 1986;85(4):287-9. doi: 10.1007/BF00493478.
The immunocytochemical localization of 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) in porcine testes was examined by applying an indirect-immunofluorescence method using an antiporcine testicular 17 beta-HSD antibody. Only the Leydig cells located in the interstitial tissue exhibited a positive immunoreaction for 17 beta-HSD: the germ cells and Sertoli cells located in the seminiferous tubules were entirely negative. These results suggest that, in porcine testis, the biosynthesis of testicular testosterone, the final step of which is the conversion of androstenedione to testosterone, takes place in the Leydig cells.
运用间接免疫荧光法，使用抗猪睾丸17β-羟类固醇脱氢酶（17β-HSD）抗体，检测了猪睾丸中17β-羟类固醇脱氢酶（17β-HSD）的免疫细胞化学定位。仅位于间质组织中的睾丸间质细胞对17β-HSD呈现阳性免疫反应：位于生精小管中的生殖细胞和支持细胞均呈阴性。这些结果表明，在猪睾丸中，睾丸睾酮的生物合成（其最后一步是将雄烯二酮转化为睾酮）发生在睾丸间质细胞中。
