School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Int J Mol Sci. 2018 Aug 31;19(9):2590. doi: 10.3390/ijms19092590.
The application of whole cells as catalytic biofilms in microchannels has attracted increasing scientific interest. However, the excessive biomass formation and structure of biofilms in a reactor limits their use. A microchannel reactor with surface modification was used to colonize recombinant BL21-pET28a- rapidly and accelerated growth of biofilms in the microchannel. The segmented flow system of 'air/culture medium containing nanomaterials' was firstly used to modulate the biofilms formation of recombinant ; the inhibitory effects of nanomaterials on biofilm formation were investigated. The results indicated that the segmental flow mode has a significant impact on the structure and development of biofilms. Using the channels modified by silane reagent, the culture time of biofilms (30 h) was reduced by 6 h compared to unmodified channels. With the addition of graphene sheets (10 mg/L) in Luria-Bertani (LB) medium, the graphene sheets possessed a minimum inhibition rate of 3.23% against recombinant . The biofilms cultivated by the LB medium with added graphene sheets were stably formed in 20 h; the formation time was 33.33% shorter than that by LB medium without graphene. The developed method provides an efficient and simple approach for rapid preparation of catalytic biofilms in microchannel reactors.
将全细胞作为催化生物膜应用于微通道引起了越来越多的科学关注。然而，反应器中生物膜的生物量过多和结构限制了其应用。使用经过表面修饰的微通道反应器来快速定植重组 BL21-pET28a-，并加速生物膜在微通道中的生长。首次使用“含纳米材料的空气/培养基”分段流动系统来调节重组生物膜的形成；研究了纳米材料对生物膜形成的抑制作用。结果表明，分段流动模式对生物膜的结构和发展有显著影响。使用硅烷试剂修饰的通道，与未修饰的通道相比，生物膜的培养时间（30 h）缩短了 6 h。在 LB 培养基中加入石墨烯片（10 mg/L），石墨烯片对重组的最小抑制率为 3.23%。在加入石墨烯片的 LB 培养基中培养的生物膜在 20 h 内稳定形成；形成时间比没有石墨烯的 LB 培养基缩短了 33.33%。所开发的方法为在微通道反应器中快速制备催化生物膜提供了一种高效、简单的方法。
