School of Biotechnology & School of the Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212018, P.R. China.
Sericulture Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212018, P.R. China.
Sci Rep. 2017 Jun 27;7(1):4309. doi: 10.1038/s41598-017-04216-4.
Enzymatic catalysis in microreactors has attracted growing scientific interest because of high specific surface enabling heat and mass transfer and easier control of reaction parameters in microreactors. However, two major challenges that limit their application are fast inactivation and the inability to the biocatalysts in microchannel reactors. A fluid and unsinkable immobilized enzyme were firstly applied in a microchannel reactor for biocatalysis in this study. Functionalized forms of graphene-immobilized naringinase flowing in microchannels have yielded excellent results for isoquercitrin production. A maximum yield of 92.24 ± 3.26% was obtained after 20 min in a microchannel reactor. Ten cycles of enzymatic hydrolysis reaction were successively completed and an enzyme activity above 85.51 ± 2.76% was maintained. The kinetic parameter V /K increased to 1.9-fold and reaction time was decreased to 1/3 compared with that in a batch reactor. These results indicated that the moving and unsinkable graphene sheets immobilized enzyme with a high persistent specificity and a mild catalytic characteristic enabled the repetitive use of enzyme and significant cost saving for the application of enzyme catalysis. Thus, the developed method has provided an efficient and simple approach for the productive and repeatable microfluidic biocatalysis.
在微反应器中进行的酶催化由于具有高比表面积，能够实现热传递和质量传递，并且更容易控制微反应器中的反应参数，因此引起了越来越多的科学关注。然而，限制其应用的两个主要挑战是酶的快速失活和无法在微通道反应器中固定生物催化剂。在这项研究中，首次将可流动且不沉的固定化酶应用于微通道反应器中的生物催化。在微通道中流动的功能化石墨烯固定化柚皮苷酶形式已在异槲皮苷生产中取得了优异的效果。在微通道反应器中经过 20 分钟后，获得了 92.24 ± 3.26%的最大产量。成功完成了 10 个循环的酶水解反应，并且保持了 85.51 ± 2.76%以上的酶活性。与分批式反应器相比，V / K 值增加了 1.9 倍，反应时间缩短了 1/3。这些结果表明，可移动且不沉的石墨烯片固定化酶具有高持续特异性和温和的催化特性，使酶能够重复使用，并为酶催化的应用节省了大量成本。因此，所开发的方法为生产性和可重复的微流控生物催化提供了一种高效且简单的方法。
