Department of Chemistry and Biochemistry, 236 Nieuwland Science Hall, University of Notre Dame, Notre Dame, IN 46556, USA.
Molecules. 2018 Sep 1;23(9):2229. doi: 10.3390/molecules23092229.
A new family of fluorescent thiophene and thienothiophene-containing squaraine dyes is described with tunable wavelengths that cover the absorption/emission range of 600⁻800 nm. The deep-red and near-infrared fluorescent compounds were easily prepared by simple synthesis and purification methods. Spectral studies showed that each squaraine was rapidly encapsulated by a tetralactam macrocycle, with nanomolar affinity in water, to produce a threaded supramolecular complex with high chemical stability, increased fluorescence quantum yield, and decreased fluorescence quenching upon dye self-aggregation. Energy transfer within the supramolecular complex permitted multiplex emission. That is, two separate dyes with fluorescence emission bands that match the popular Cy5 and Cy7 channels, could be simultaneously excited with a beam of 375 nm light. A broad range of practical applications is envisioned in healthcare diagnostics, microscopy, molecular imaging, and fluorescence-guided surgery.
描述了一类新型的含有噻吩和噻吩并噻吩的荧光方酸染料，它们的波长可调，涵盖了 600nm-800nm 的吸收/发射范围。这些深红光和近红外荧光化合物可以通过简单的合成和纯化方法轻松制备。光谱研究表明，每个方酸都可以通过四元内酰胺大环快速包裹，在水中具有纳摩尔亲和力，从而产生具有高化学稳定性、高荧光量子产率和染料自聚集时荧光猝灭降低的螺旋超分子复合物。超分子复合物内的能量转移允许多重发射。也就是说，可以用 375nm 的光束同时激发具有匹配流行的 Cy5 和 Cy7 通道的荧光发射带的两种单独的染料。在医疗保健诊断、显微镜、分子成像和荧光引导手术等方面有广泛的实际应用。
