硝酸盐在菠菜中的积累及其参与硝酸盐运输和同化的基因表达模式。
Nitrate Accumulation and Expression Patterns of Genes Involved in Nitrate Transport and Assimilation in Spinach.
机构信息
Development and Collaborative Innovation Center of Plant Germplasm Resources, Shanghai Engineering Research Center of Plant Germplasm Resources, College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China.
出版信息
Molecules. 2018 Sep 2;23(9):2231. doi: 10.3390/molecules23092231.
Excessive accumulation of nitrate in spinach is not only harmful to human beings, but also limits the efficiency of nitrogen usage. However, the underlying mechanism of nitrate accumulation in plants remains unclear. This study analyzed the physiological and molecular characteristics of nitrate uptake and assimilation in the spinach varieties with high or low nitrate accumulation. Our results showed that the variety of spinach with a high nitrate content (So18) had higher nitrate uptake compared to the variety with a low nitrate content (So10). However, the nitrate reductase activities of both varieties were similar, which suggests that the differential capacity to uptake and transport nitrate may account for the differences in nitrate accumulation. The quantitative PCR analysis showed that there was a higher level of expression of spinach nitrate transporter genes in So18 compared to those in So10. Based on the function of Arabidopsis homologs , the role of spinach in nitrate accumulation is discussed. It is concluded that further work focusing on the expression of (especially for , and ) may help us to elucidate the molecular mechanism of nitrate accumulation in spinach.
菠菜中硝酸盐的过度积累不仅对人类有害，还会限制氮的利用效率。然而，硝酸盐在植物中积累的机制尚不清楚。本研究分析了高、低硝酸盐积累菠菜品种硝酸盐吸收和同化的生理和分子特性。结果表明，高硝酸盐含量（So18）的菠菜品种的硝酸盐吸收量高于低硝酸盐含量（So10）的品种。然而，两种品种的硝酸还原酶活性相似，这表明硝酸盐的吸收和转运能力的差异可能是硝酸盐积累差异的原因。定量 PCR 分析表明，So18 中菠菜硝酸盐转运基因的表达水平高于 So10。根据拟南芥同源物的功能，讨论了菠菜在硝酸盐积累中的作用。结论是，进一步关注（特别是 、 和 ）的表达可能有助于阐明菠菜硝酸盐积累的分子机制。
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