Lin Xiao-Min, Wu Ze-Xin, Lai Jie-Qing, Tao Hao-Lin, Wei Wen-Hui, Zhuang Hui-Ling, Chen Yang, Lian Da-Wei
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510000, China.
First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510000, China.
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(15):3171-3175. doi: 10.19540/j.cnki.cjcmm.20180608.001.
The aim of this paper is to investigate the effect of patchouli alcohol in enhancing Helicobater pylori's action in eradicating macrophages and its mechanism. H. pylori was co-cultured with macrophages at a ratio of MOI=100 in different concentrations of patchouli alcohol. The effect of patchouli alcohol in eradicating macrophages was detected by agar dilution method. The effect of patchouli alcohol on NO and myeloperoxidase (MPO) levels in macrophages were measured by H. pylori by biochemical methods. Patchouli alcohol effect on H. pylori-induced pro-inflammatory gene expression and protein secretion in macrophages were detected by RT-qPCR and ELISA method. The eradication of H. pylori has significantly enhanced, and the destabilization of lysosomes has been reversed. Meanwhile, patchouli alcohol has an effect in inhibiting pro-inflammation and oxidation. The mechanism of patchouli alcohol in eradicating H. pylori and resisting oxidative stress may be associated to the blocking of bacteria escape lysosome combination procedures.
本文旨在研究广藿香醇增强幽门螺杆菌清除巨噬细胞的作用及其机制。将幽门螺杆菌与巨噬细胞以感染复数（MOI）=100的比例在不同浓度的广藿香醇中共同培养。采用琼脂稀释法检测广藿香醇清除巨噬细胞的效果。通过生化方法检测广藿香醇对幽门螺杆菌诱导的巨噬细胞中一氧化氮（NO）和髓过氧化物酶（MPO）水平的影响。采用逆转录-定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）法检测广藿香醇对幽门螺杆菌诱导的巨噬细胞促炎基因表达和蛋白分泌的影响。幽门螺杆菌的清除显著增强，溶酶体的不稳定状态得到逆转。同时，广藿香醇具有抑制炎症和氧化的作用。广藿香醇清除幽门螺杆菌和抵抗氧化应激的机制可能与阻断细菌逃逸溶酶体的结合过程有关。
