Sakamoto Y, Miyazaki T, Kai M, Ohkura Y
J Chromatogr. 1986 Aug 2;380(2):313-20. doi: 10.1016/s0378-4347(00)83659-2.
A high-performance liquid chromatographic method is described for the assay of angiotensin-converting enzyme in human serum and for the separation of angiotensins and their analogues after pre-column fluorescence derivatization with benzoin. Angiotensin II, formed enzymatically from angiotensin I, is converted into a fluorescent derivative which is then separated isocratically from the substrate and biological substances in the enzyme reaction mixture on a reversed-phase column (TSK gel ODS-120T). The lower limit of detection for angiotensin II is 0.66 pmol per enzyme assay tube. The method is simple and sensitive, and requires as little as 5 microliter of human serum. Angiotensin analogues can also be separated and quantified by the chromatographic technique, and thus this method permits the use of the analogues of angiotensin I as substrates.
本文描述了一种高效液相色谱法，用于测定人血清中的血管紧张素转换酶，以及在使用安息香进行柱前荧光衍生化后分离血管紧张素及其类似物。由血管紧张素I酶促形成的血管紧张素II被转化为荧光衍生物，然后在反相柱（TSK凝胶ODS - 120T）上与酶反应混合物中的底物和生物物质进行等度分离。每个酶分析管中血管紧张素II的检测下限为0.66皮摩尔。该方法简单且灵敏，仅需5微升人血清。血管紧张素类似物也可通过色谱技术进行分离和定量，因此该方法允许使用血管紧张素I的类似物作为底物。
