Badminton M N, Dawson C M, Rainbow S J, Tickner T R
Department of Chemical Pathology, Norfolk and Norwich Hospital, UK.
Ann Clin Biochem. 1991 Jul;28 ( Pt 4):396-400. doi: 10.1177/000456329102800415.
A non-extraction high performance liquid chromatography assisted method for the measurement of angiotensin-converting enzyme in serum has been developed. Serum containing the enzyme was incubated with the synthetic substrate, furylacryloylphenylalanylglycylglycine at a concentration approaching 10 times the apparent Km, prior to injection onto a shielded hydrophobic reverse phase high performance liquid chromatography column. This allowed the product to be separated and measured directly without the need for a time consuming protein extraction step. The method is enzymatically sound and avoids the methodological problems associated with automated kinetic assays.
已开发出一种用于测量血清中血管紧张素转换酶的非萃取高效液相色谱辅助方法。在将含有该酶的血清注入屏蔽的疏水反相高效液相色谱柱之前，先将其与合成底物呋喃丙烯酰苯丙氨酰甘氨酰甘氨酸以接近表观Km值10倍的浓度进行孵育。这使得产物能够直接分离和测量，无需耗时的蛋白质提取步骤。该方法在酶学上是合理的，并且避免了与自动动力学测定相关的方法学问题。
