Chen Liang-Yuan, Cheng Xuan-Xuan, Tang Xiao-Min, Yang Quan
Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station of National Industrial Technology System for Chinese Materia Medica, Guangdong Engineering Research Center of Good Agricultural Practice & Comprehensive Development for Cantonese Medicinal Materials, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(16):3322-3328. doi: 10.19540/j.cnki.cjcmm.2018.0097.
HPLC analysis was performed on a Phenomenex PS C₁₈（4.6 mm×250 mm, 5 μm）column using methanol -0.2% formic acid (30:70) at a flow rate of 0.8 mL·min⁻¹. The column temperature was 30 °C and the detection wavelength was set at 335 nm. The injection volume was 10 μL. The HPLC fingerprint of Desmodium styracifolium was established with 10 common peaks, and 5 of them were identified as vicenin-1, schaftoside, isoorientin, isoschaftoside and isovitexin, respecivetly. The fingerprints of 21 batches of D. styracifolium samples were analyzed with similarity evaluation, cluster analysis, principal component analysis and partial least squares discriminant analysis. There was no significant difference among the quantitative results of these five ingredients verified by external standard method (ESM) and quantitative analysis of multi-components by single marker (QAMS) method. The application of fingerprint, pattern recognition combined with QAMS can provide more comprehensive references for the quality control and evaluation of D. styracifolium.
采用Phenomenex PS C₁₈（4.6 mm×250 mm, 5 μm）色谱柱进行高效液相色谱（HPLC）分析，流动相为甲醇 - 0.2%甲酸（30:70），流速为0.8 mL·min⁻¹。柱温为30℃，检测波长设定为335 nm。进样量为10 μL。建立了广金钱草的HPLC指纹图谱，共有10个共有峰，其中5个分别鉴定为三叶豆苷、schaftoside、异荭草苷、异schaftoside和异牡荆素。对21批广金钱草样品的指纹图谱进行了相似度评价、聚类分析、主成分分析和偏最小二乘判别分析。外标法（ESM）和一测多评法（QAMS）对这5种成分定量结果无显著差异。指纹图谱、模式识别结合QAMS的应用可为广金钱草的质量控制和评价提供更全面的参考。
