Zhang Yan, Hu Min, Liu Liu, Cheng Xiao-Ling, Cai Jing, Zhou Jian, Wang Tao
Department of Obstetrics and Gynaecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
J BUON. 2018 May-Jun;23(3):763-768.
The main objective of the present research work was to study the anticancer properties of rosmarinic acid in OVCAR-3 human ovarian cancer cells and also to evaluate its effects on apoptosis induction, cancer cell migration and modulation of lncRNA MALAT-1 expression.
MTT assay was used to study the effects of the agent on OVCAR-3 cell viability, while inverted phase contrast microscopy and fluorescence microscopy were used to study the effects on cell morphology. Scanning electron microscopy (SEM) was used to study the effects of rosmarinic acid on cell surface morphology in OVCAR-3 cells. In vitro wound healing assay was used to study the effects on cell migration.
Rosmarinic acid induced time-dependent and concentration- dependent cytotoxic effects in these malignant cells. The IC50 values at 48 and 72 hrs time intervals were found to be 34.6 and 25.1 μM respectively. Rosmarinic acidtreated cells revealed significant changes in cell morphology including cellular shrinkage and cell rounding. The cells also lost attachment with the plate surface. Doses of 10, 40 and 160 μM rosmarinic acid led to a substantial increase in bright blue fluorescence which is a signpost of chromatin condensation and DNA fragmentation. Rosmarinic acid treatment also led to a significant suppression of cell migration corresponding to 46.5% and 86.2 % cell migration inhibition at 40 and 160 μM doses, respectively.
In conclusion, the current study showed that rosmarinic acid induced potent anticancer effects in OCVAR- 3 cancer cells by inducing apoptosis, inhibiting cell migration and modulating lncRNA Malat-1 expression.
本研究工作的主要目的是研究迷迭香酸对OVCAR-3人卵巢癌细胞的抗癌特性，并评估其对诱导细胞凋亡、癌细胞迁移以及长链非编码RNA MALAT-1表达调控的影响。
采用MTT法研究该药物对OVCAR-3细胞活力的影响，同时利用倒置相差显微镜和荧光显微镜研究其对细胞形态的影响。使用扫描电子显微镜（SEM）研究迷迭香酸对OVCAR-3细胞表面形态的影响。采用体外伤口愈合试验研究其对细胞迁移的影响。
迷迭香酸在这些恶性细胞中诱导了时间和浓度依赖性的细胞毒性作用。发现在48小时和72小时时间间隔的IC50值分别为34.6和25.1μM。经迷迭香酸处理的细胞显示出细胞形态的显著变化，包括细胞皱缩和变圆。细胞也失去了与平板表面的附着。10、40和160μM剂量的迷迭香酸导致亮蓝色荧光大幅增加，这是染色质浓缩和DNA片段化的标志。迷迭香酸处理还导致细胞迁移受到显著抑制，在40和160μM剂量下分别对应46.5%和86.2%的细胞迁移抑制率。
总之，当前研究表明迷迭香酸通过诱导细胞凋亡、抑制细胞迁移和调控长链非编码RNA Malat-1表达，在OCVAR-3癌细胞中诱导了强大的抗癌作用。
