Institute for Bioengineering Research, School of Engineering, University of Kansas, 1530 W. 15th Street, Lawrence, KS 66045-7609, USA.
Institute for Bioengineering Research, School of Engineering, University of Kansas, 1530 W. 15th Street, Lawrence, KS 66045-7609, USA; Department of Civil Engineering, University of Kansas, 1530 W. 15th Street, Lawrence, KS 66045-7609, USA.
Dent Mater. 2018 Nov;34(11):1652-1660. doi: 10.1016/j.dental.2018.08.293. Epub 2018 Sep 7.
The objective of this study was to explore the effect of lysine integration to dental adhesives with respect to the polymerization kinetics, neutralization capacities in the acidic microenvironment, dynamic mechanical properties, and thermal properties.
Lysine was incorporated into liquid resin formulations at 2.5 and 5.0wt % with additional water/ethanol co-solvents. The co-monomer system contained 2-hydroxyethyl-methacrylate (HEMA) and Bisphenol A glycerolate dimethacrylate (BisGMA) with a mass ratio of 45/55. The kinetics of photopolymerization, neutralization capacities, lysine-leaching, dynamic mechanical properties and thermal properties of the control and experimental adhesives were analyzed.
The degree of conversion of the experimental adhesive was increased substantially at 2.5wt% lysine as compared to the control. The experimental polymers provided acute neutralization of the acidic microenvironment. Approximately half of the lysine was released from the polymer network within one month. Under dry conditions and physiologic temperatures, the incorporation of lysine did not compromise the storage modulus. Comparison of the thermal properties suggests that the more compact structure of the control adhesive inhibits movement of the polymer chains resulting in increased T.
Incorporating lysine in the adhesive formulations led to promising results regarding modulating pH, which may serve as one aspect of a multi-spectrum approach for enhancing the durability of composite restorations. The results provide insight and lay a foundation for incorporating amino acids or peptides into adhesive formulations for pH modulation or desired bioactivity at the interfacial margin between the composite and tooth.
本研究旨在探讨赖氨酸整合到牙科胶粘剂中的效果，具体涉及聚合动力学、酸性微环境中的中和能力、动态力学性能和热性能。
赖氨酸以 2.5 和 5.0wt%的比例加入到液体树脂配方中，并添加水/乙醇共溶剂。共聚单体系统包含 2-羟乙基甲基丙烯酸酯（HEMA）和双酚 A 甘油二甲基丙烯酸酯（BisGMA），质量比为 45/55。分析了对照和实验性胶粘剂的光聚合动力学、中和能力、赖氨酸浸出、动态力学性能和热性能。
与对照相比，2.5wt%赖氨酸的实验性胶粘剂的转化率显著提高。实验性聚合物对酸性微环境提供了急剧的中和作用。大约一半的赖氨酸在一个月内从聚合物网络中释放出来。在干燥条件和生理温度下，赖氨酸的掺入并未降低储存模量。对热性能的比较表明，对照胶粘剂的更紧密结构抑制了聚合物链的运动，从而导致 T 增加。
将赖氨酸纳入胶粘剂配方中，在调节 pH 值方面取得了有希望的结果，这可能是增强复合材料修复体耐久性的多谱方法的一个方面。研究结果提供了深入的见解，并为在复合材料和牙齿之间的界面边缘将氨基酸或肽纳入胶粘剂配方以调节 pH 值或达到所需的生物活性奠定了基础。
