Dept. of Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Dept. of Scienze della Vita e dell'Ambiente, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Int J Pharm. 2018 Nov 15;551(1-2):34-41. doi: 10.1016/j.ijpharm.2018.09.009. Epub 2018 Sep 7.
Transfersomes were prepared by using different polysorbates (i.e., Tween 20, 40, 60 and 80) and loaded with tocopherol acetate, a naturally-occurring phenolic compound with antioxidant activity. The vesicles showed unilamellar morphology, small size (∼85 nm), low polydispersity index (≤0.27), and high entrapment efficiency, which increased as a function of the length of the Tween fatty acid chain (from 72% to 90%). The long-term stability of the formulations was evaluated by means of the Turbiscan™ technology, which indicated their good stability, irrespective of the Tween used. The vesicles efficiently delivered tocopherol to the skin, and showed biocompatibility in vitro in keratinocytes and fibroblasts. Regardless of the Tween used, the transfersomes were able to protect skin cells from the oxidative damage induced by hydrogen peroxide. Additionally, transfersomes promoted cell proliferation and migration, which resulted in an acceleration of skin wound closure. These results demonstrated that tocopherol-loaded transfersomes bear potential as topical delivery system with antioxidant activity and wound healing properties.
传递体通过使用不同的聚山梨醇酯（即吐温 20、40、60 和 80）来制备，并负载有醋酸生育酚，这是一种具有抗氧化活性的天然酚类化合物。这些囊泡呈现单分子层形态，粒径小（约 85nm），多分散指数低（≤0.27），包封效率高，且随着吐温脂肪酸链长度的增加而增加（从 72%增加到 90%）。通过 Turbiscan™技术评估了配方的长期稳定性，结果表明无论使用哪种吐温，其稳定性都很好。这些囊泡能够有效地将生育酚递送到皮肤中，并在角质形成细胞和成纤维细胞中表现出体外生物相容性。无论使用哪种吐温，传递体都能够保护皮肤细胞免受过氧化氢诱导的氧化损伤。此外，传递体还促进细胞增殖和迁移，从而加速皮肤伤口愈合。这些结果表明，负载生育酚的传递体具有作为具有抗氧化活性和伤口愈合特性的局部递送系统的潜力。
