Tsutsumi C, Hoshiba K, Moriuchi S, Orimo H, Hosoya N
J Nutr Sci Vitaminol (Tokyo). 1986 Apr;32(2):157-69. doi: 10.3177/jnsv.32.157.
Regulatory effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2-D3), 24,25-dihydroxyvitamin D3 (24,25-(OH)2-D3), parathyroid hormone (PTH) and calcitonin (CT) on BGP synthesis and/or secretion were studied using chick embryonic calvaria in vitro. BGP contents in calvaria and culture medium were determined by radioimmunoassay using antiserum to purified chick BGP. After 72 h culture, 1,25-(OH)2-D3 (10(-10)-10(-7) M) increased the BGP content in culture medium significantly, and the effect was maximum at 10(-8)M, while the BGP content in calvaria was not changed by 1,25-(OH)2-D3. 24,25-(OH)2-D3 increased the BGP content both in medium at 10(-6) M-10(-5) M after 24 h culture and in calvaria at 5 X 10(-7)-10(-5) M after 48 h culture. PTH increased BGP contents both in calvaria after 72 h culture and medium at 1 U/ml after 24 h culture, while it decreased them at 5-10 U/ml after 24 h culture. CT (0.5-10 U/ml) had no effect on BGP contents in calvaria and medium. 1,25-(OH)2-D3-induced increase of BGP in culture medium was observed from 24 to 72 h of culture and then reached a plateau at 120 h of culture. 1,25-(OH)2-D3-induced increase in the BGP level in medium after 72 h culture was 3-4 times the control value. On the other hand, the BGP content in calvaria was not affected by 1,25-(OH)2-D3 until 72 h of culture and then significantly increased at 120 h. The addition of 1,25-(OH)2-D3 (10(-8) M), 24,25-(OH)2-D3 (10(-6) M), and 1 U PTH (and 1 U CT added to those hormones) into culture medium of chick embryonic calvaria significantly increased BGP contents in calvaria and medium at 72 h after culture. The effect of three hormones was not synergistic, but had a tendency to be greater than that of a single addition of 1,25-(OH)2-D3, 24,25-(OH)2-D3 or PTH. Further addition of 1 U CT into culture medium did not affect the combined effects of 1,25-(OH)2-D3, 24,25-(OH)2-D3 and PTH except for the calcium content in calvaria. The addition of individual calcium-regulating hormones into culture medium of chick embryonic calvaria did not affect the calcium and phosphorus contents in calvaria, but the calcium content in calvaria was significantly increased by the addition of 1,25-(OH)2-D3, 24,25-(OH)2-D3 and PTH to the culture medium.(ABSTRACT TRUNCATED AT 400 WORDS)
采用鸡胚颅骨体外培养模型，研究了1,25 - 二羟维生素D3（1,25-(OH)2-D3）、24,25 - 二羟维生素D3（24,25-(OH)2-D3）、甲状旁腺激素（PTH）及降钙素（CT）对骨钙素（BGP）合成和/或分泌的调节作用。采用抗纯化鸡BGP抗血清，通过放射免疫分析法测定颅骨和培养基中的BGP含量。培养72小时后，1,25-(OH)2-D3（10(-10)-10(-7)M）显著增加培养基中的BGP含量，在10(-8)M时作用最强，而1,25-(OH)2-D3对颅骨中的BGP含量无影响。培养24小时后，24,25-(OH)2-D3在10(-6)M - 10(-5)M时增加培养基中的BGP含量，培养48小时后，在5×10(-7)-10(-5)M时增加颅骨中的BGP含量。培养72小时后，PTH增加颅骨中的BGP含量，培养24小时后，1U/ml的PTH增加培养基中的BGP含量，但培养24小时后，5 - 10U/ml的PTH降低BGP含量。CT（0.5 - 10U/ml）对颅骨和培养基中的BGP含量无影响。培养24至72小时观察到1,25-(OH)2-D3诱导培养基中BGP增加，培养120小时达到平台期。培养72小时后，1,25-(OH)2-D3诱导培养基中BGP水平增加至对照值的3 - 4倍。另一方面，培养72小时前，1,25-(OH)2-D3对颅骨中的BGP含量无影响，培养120小时后显著增加。向鸡胚颅骨培养基中添加1,25-(OH)2-D3（10(-8)M）、24,25-(OH)2-D3（10(-6)M）和1U PTH（并向这些激素中添加1U CT），培养72小时后显著增加颅骨和培养基中的BGP含量。三种激素的作用并非协同作用，但趋势是大于单独添加1,25-(OH)2-D3、24,25-(OH)2-D3或PTH的作用。向培养基中进一步添加1U CT，除了颅骨中的钙含量外，不影响1,25-(OH)2-D3、24,25-(OH)2-D3和PTH的联合作用。向鸡胚颅骨培养基中单独添加钙调节激素不影响颅骨中的钙和磷含量，但向培养基中添加1,25-(OH)2-D3、24,25-(OH)2-D3和PTH可显著增加颅骨中的钙含量。（摘要截短于400字）
