Brommage R, Hart L E, DeLuca H F
Experientia. 1986 May 15;42(5):553-4. doi: 10.1007/BF01946699.
Chick embryos from vitamin D-deficient hens given physiological doses of 1,25-dihydroxyvitamin D3 or 24,25-dihydroxyvitamin D3 or both become severely hypocalcemic, hyperphosphatemic and fail to hatch as compared to those derived from hens given 25-hydroxyvitamin D3 or 24,25-difluoro-25-hydroxyvitamin D3. Calvariae from the former contain less mineral and on incubation in vitro produce significantly lower calcium and higher phosphate concentration in the medium than do the calvariae derived from the embryos of hens supported on 25-hydroxyvitamin D3 or 24,24-difluoro-25-hydroxyvitamin D3.
与来自给予25-羟基维生素D3或24,25-二氟-25-羟基维生素D3的母鸡的胚胎相比，给予生理剂量的1,25-二羟基维生素D3或24,25-二羟基维生素D3或两者的维生素D缺乏母鸡的鸡胚会出现严重的低钙血症、高磷血症，并且无法孵化。前者的颅骨所含矿物质较少，与来自给予25-羟基维生素D3或24,24-二氟-25-羟基维生素D3的母鸡胚胎的颅骨相比，在体外培养时，其培养基中产生的钙浓度显著降低，磷浓度升高。
