Ammar Mohamed M, Waly Gihan H, Saniour Sayed H, Moussa Taheya A
Biomaterials Department, Faculty of Oral and Dental Medicine, Future University, Cairo, Egypt.
Biomaterials Department, Faculty of Dentistry, Cairo University, Cairo, Egypt.
Saudi Dent J. 2018 Oct;30(4):355-364. doi: 10.1016/j.sdentj.2018.06.002. Epub 2018 Jun 27.
Periodontium regeneration is a highly challenging process as it requires the regeneration of three different tissues simultaneously. The aim of this study was to develop a composite material that can be easily applied and can sufficiently deliver essential growth factors and progenitor cells for periodontal tissue regeneration. Freeze-dried platelet concentrate (FDPC) was prepared and incorporated in a thermo-sensitive chitosan/β-glycerol phosphate (β-GP) hydrogel at concentrations of 5, 10, or 15 mg/ml. The viscosity of the hydrogels was investigated as the temperature rises from 25 °C to 37 °C and the release kinetics of transforming growth factor (TGF-β1), platelet-derived growth factor (PDGF-BB) and insulin-like growth factor (IGF-1) were investigated at four time points (1 h, 1 day, 1 week, 2 weeks). Periodontal ligament stem cells (PDLSCs) were isolated from human third molars and encapsulated in the different hydrogel groups. Their viability was investigated after 7 days in culture in comparison to standard culture conditions and non FDPC-loaded hydrogel. Results showed that loading FDPC in the hydrogel lowered the initial viscosity in comparison to the unloaded control group and did not affect the sol-gel transition in any group. All FDPC-loaded hydrogel groups exhibited sustained release of TGF-β1 and PDGF-BB for two weeks with significant difference between the different concentrations. The loading of 10 and 15 mg/ml of FDPC in the hydrogel increased the PDLSCs viability significantly compared to the unloaded hydrogel and was comparable to the standard culture conditions. Accordingly, it may be concluded that loading FDPC in a chitosan/β-GP hydrogel can offer enhanced injectability, a sustained release of growth factors and increased viability of encapsulated stem cells which can be beneficial in periodontium tissue regeneration.
牙周组织再生是一个极具挑战性的过程，因为它需要同时再生三种不同的组织。本研究的目的是开发一种复合材料，这种材料易于应用，并且能够充分递送用于牙周组织再生的必需生长因子和祖细胞。制备了冻干血小板浓缩物（FDPC），并以5、10或15 mg/ml的浓度掺入热敏壳聚糖/β-甘油磷酸酯（β-GP）水凝胶中。研究了水凝胶在温度从25°C升高到37°C时的粘度，并在四个时间点（1小时、1天、1周、2周）研究了转化生长因子（TGF-β1）、血小板衍生生长因子（PDGF-BB）和胰岛素样生长因子（IGF-1）的释放动力学。从人第三磨牙中分离出牙周膜干细胞（PDLSCs），并将其封装在不同的水凝胶组中。与标准培养条件和未加载FDPC的水凝胶相比，在培养7天后研究了它们的活力。结果表明，与未加载的对照组相比，在水凝胶中加载FDPC降低了初始粘度，并且在任何组中都不影响溶胶-凝胶转变。所有加载FDPC的水凝胶组在两周内均表现出TGF-β1和PDGF-BB的持续释放，不同浓度之间存在显著差异。与未加载的水凝胶相比，在水凝胶中加载10和15 mg/ml的FDPC显著提高了PDLSCs的活力，并且与标准培养条件相当。因此，可以得出结论，在壳聚糖/β-GP水凝胶中加载FDPC可以提供增强的可注射性、生长因子的持续释放以及封装干细胞活力的增加，这对牙周组织再生可能是有益的。
