CRAC通道快速钙依赖性失活（FCDI）的测量。

Measurement of the CRAC Channel Fast Ca-Dependent Inactivation (FCDI).

作者信息

Rychkov Grigori Y

机构信息

School of Medicine, University of Adelaide, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA, Australia.

出版信息

Methods Mol Biol. 2018;1843:167-173. doi: 10.1007/978-1-4939-8704-7_14.

DOI:10.1007/978-1-4939-8704-7_14

PMID:30203286

Abstract

Fast Ca-dependent inactivation (FCDI) is a safety mechanism limiting Ca entry through some types of Ca channels, including Ca release-activated Ca (CRAC) channels. This type of inactivation is caused by Ca, which passes through Ca channel and binds to a specific site within a short distance from the inner mouth of the pore, causing channel to shut.The main technique that is used to investigate FCDI is whole-cell patch clamping. Since the cloning of the molecular components of the CRAC channel, STIM1 and Orai1, FCDI of CRAC channel has been studied using HEK293T heterologous expression system. In this paper we describe a method of quantifying CRAC channel FCDI by using instantaneous tail currents.

摘要

快速钙依赖性失活（FCDI）是一种安全机制，可限制钙通过某些类型的钙通道进入细胞，包括钙释放激活钙（CRAC）通道。这种类型的失活是由钙引起的，钙通过钙通道并在距孔内口短距离内与特定位点结合，导致通道关闭。用于研究FCDI的主要技术是全细胞膜片钳技术。自从克隆出CRAC通道的分子成分STIM1和Orai1以来，已使用HEK293T异源表达系统研究了CRAC通道的FCDI。在本文中，我们描述了一种通过使用瞬时尾电流来量化CRAC通道FCDI的方法。