Microbial Sciences Institute, Yale University, West Haven, CT, 06516, USA.
Department of Chemistry, Yale University, New Haven, CT, 06511, USA.
Curr Top Microbiol Immunol. 2019;420:73-91. doi: 10.1007/82_2018_129.
Activity-based protein profiling (ABPP) is a technique for selectively detecting reactive amino acids in complex proteomes with the aid of chemical probes. Using probes that target catalytically active enzymes, ABPP can rapidly define the functional proteome of a biological system. In recent years, this approach has been increasingly applied to globally profile enzymes active at the host-pathogen interface of microbial infections. From in vitro co-culture systems to animal models of infection, these studies have revealed enzyme-mediated mechanisms of microbial pathogenicity, host immunity, and metabolic adaptation that dynamically shape pathogen interactions with the host.
活性蛋白质组学分析（ABPP）是一种利用化学探针选择性检测复杂蛋白质组中反应性氨基酸的技术。使用靶向催化活性酶的探针，ABPP 可以快速定义生物系统的功能蛋白质组。近年来，这种方法已越来越多地应用于微生物感染宿主-病原体界面的酶的全局分析。从体外共培养系统到感染动物模型，这些研究揭示了酶介导的微生物致病性、宿主免疫和代谢适应机制，这些机制动态地塑造了病原体与宿主的相互作用。
