Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.
Jiangsu Frey Agrochemicals Co. Ltd, Lianyungang, Jiangsu, 222005, China.
J Ind Microbiol Biotechnol. 2018 Dec;45(12):1033-1044. doi: 10.1007/s10295-018-2076-7. Epub 2018 Sep 10.
Bacillus velezensis B006 is a biocontrol agent which functions through effective colonization and surfactin production. To reveal the surfactin-producing mechanism, gas chromatography-mass spectrometry based untargeted metabolomics was performed to compare the metabolite profiles of strain B006 grown in industrial media M3 and M4. Based on the statistical and pathway topology analyses, a total of 31 metabolites with a fold change of less than - 1.0 were screened as the significantly altered metabolites, which distributed in 15 metabolic pathways. Fourteen amino acids involving in the metabolisms of alanine/aspartate/glutamate, glycine/serine/threonine, arginine/proline, glutathione/cysteine/methionine and valine/leucine/isoleucine as well as succinic acid in TCA cycle were identified to be the hub metabolites. Aminoacyl-tRNA biosynthesis, glycerolipid metabolism, and pantothenate/CoA biosynthesis also contributed to surfactin production. To the best of our knowledge, this study is the first to investigate the metabolic pathways of B. velezensis on surfactin production, and will benefit the optimization of commercial fermentation for higher surfactin yield.
解淀粉芽孢杆菌 B006 是一种通过有效定殖和表面活性剂生产起作用的生物防治剂。为了揭示表面活性剂的产生机制，采用基于气相色谱-质谱的非靶向代谢组学方法比较了菌株 B006 在工业培养基 M3 和 M4 中生长的代谢物图谱。基于统计和途径拓扑分析，共筛选出 31 种丰度变化小于-1.0 的代谢物作为显著变化的代谢物，分布在 15 种代谢途径中。鉴定出包括丙氨酸/天冬氨酸/谷氨酸、甘氨酸/丝氨酸/苏氨酸、精氨酸/脯氨酸、谷胱甘肽/半胱氨酸/蛋氨酸和缬氨酸/亮氨酸/异亮氨酸以及三羧酸循环中的琥珀酸代谢在内的 14 种氨基酸以及酰基-tRNA 生物合成、甘油脂代谢和泛酸/辅酶 A 生物合成对表面活性剂的产生也有贡献。据我们所知，这项研究首次调查了解淀粉芽孢杆菌在表面活性剂生产上的代谢途径，将有助于优化商业发酵以获得更高的表面活性剂产量。
