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Purified Saccharomyces cerevisiae RNA polymerase II interacts homologously with two different promoters as revealed by P1 endonuclease analysis.

作者信息

Camilloni G, Della Seta F, Grazia Ficca A, Di Mauro E

出版信息

Mol Gen Genet. 1986 Aug;204(2):249-57. doi: 10.1007/BF00425506.

DOI:10.1007/BF00425506
PMID:3020364
Abstract

The intergenic region of the Saccharomyces cerevisiae GAL1-GAL10 divergent promoters has been circularized in vitro in different topological states. In defined conditions, purified homologous RNA polymerase II forms two stable complexes (half-life approximately equal to 5 h) with this DNA in the presence of the four ribonucleotides, as determined by measurement (Gamper and Hearst 1983) of the amount and stability of the resulting unwinding. Each stable complex induces in the closed DNA domain a region of hypersensitivity to P1 endonuclease. The two induced hypersensitive regions are very similar: each maps on one promoter, spans over the 100 bp DNA sequence that encompasses the RNA Initiation Sites (RIS) and the TATA box, is composed by three subregions (one on the RIS, one proximal or overlapping the TATA sequence, one intermediate). We show that this promoter-localized interaction is supercoil-dependent.

摘要

相似文献

1
Purified Saccharomyces cerevisiae RNA polymerase II interacts homologously with two different promoters as revealed by P1 endonuclease analysis.
Mol Gen Genet. 1986 Aug;204(2):249-57. doi: 10.1007/BF00425506.
2
Structure of RNA polymerase II promoters. Conformational alterations and template properties of circularized Saccharomyces cerevisiae GAL1-GAL10 divergent promoters.RNA聚合酶II启动子的结构。酿酒酵母GAL1 - GAL10双向启动子环化后的构象改变及模板特性。
EMBO J. 1986 Apr;5(4):763-71. doi: 10.1002/j.1460-2075.1986.tb04279.x.
3
Structure of RNA polymerase II promoters. Coordinate conformational alteration of the upstream activator of the TATA- and RNA-initiation sequences under moderate torsional stress.
J Biol Chem. 1986 May 15;261(14):6145-8.
4
Topological unwinding of strong and weak promoters by RNA polymerase. A comparison between the lac wild-type and the UV5 sites of Escherichia coli.RNA聚合酶对强启动子和弱启动子的拓扑解旋。大肠杆菌lac野生型位点与UV5位点的比较。
J Mol Biol. 1987 Jun 20;195(4):795-808. doi: 10.1016/0022-2836(87)90485-2.
5
DNA melting on yeast RNA polymerase II promoters.
Science. 1993 Aug 6;261(5122):759-62. doi: 10.1126/science.8342041.
6
DNA unwinding in the CYC1 and DED1 yeast promoters.酵母CYC1和DED1启动子中的DNA解旋
Gene. 1998 Dec 28;225(1-2):153-62. doi: 10.1016/s0378-1119(98)00525-3.
7
GAL1-GAL10 divergent promoter region of Saccharomyces cerevisiae contains negative control elements in addition to functionally separate and possibly overlapping upstream activating sequences.酿酒酵母的GAL1 - GAL10发散启动子区域除了功能上独立且可能重叠的上游激活序列外，还包含负调控元件。
Genes Dev. 1987 Dec;1(10):1118-31. doi: 10.1101/gad.1.10.1118.
8
Topological properties of DNA domains and interaction with RNA polymerase II.
Basic Appl Histochem. 1987;31(3):275-80.
9
The intrinsic topological information of the wild-type and of up-promoter mutations of the Saccharomyces cerevisiae alcohol dehydrogenase II regulatory region.酿酒酵母乙醇脱氢酶II调控区野生型及上游启动子突变的内在拓扑信息。
J Biol Chem. 1988 Nov 5;263(31):15888-96.
10
An initiation element in the yeast CUP1 promoter is recognized by RNA polymerase II in the absence of TATA box-binding protein if the DNA is negatively supercoiled.如果DNA为负超螺旋状态，酵母CUP1启动子中的起始元件在没有TATA盒结合蛋白的情况下也能被RNA聚合酶II识别。
Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10745-50. doi: 10.1073/pnas.200365097.

引用本文的文献

1
Eukaryotic DNA topoisomerase I reaction is topology dependent.
Nucleic Acids Res. 1988 Jul 25;16(14B):7071-85. doi: 10.1093/nar/16.14.7071.
2
Functional relationship among TATA sequences, gene induction and transcription initiation in the beta-galactosidase, LAC4, gene from Kluyveromyces lactis.乳酸克鲁维酵母β-半乳糖苷酶基因LAC4中TATA序列、基因诱导与转录起始之间的功能关系。
Curr Genet. 1989 Apr;15(4):261-9. doi: 10.1007/BF00447041.
3
Single-stranded structures are present within plasmids containing the Epstein-Barr virus latent origin of replication.在含有爱泼斯坦-巴尔病毒潜伏性复制起点的质粒中存在单链结构。

本文引用的文献

1
Cruciform structures in supercoiled DNA.超螺旋DNA中的十字形结构。
Nature. 1981 Feb 5;289(5797):466-70. doi: 10.1038/289466a0.
2
Efficient and selective initiation by yeast RNA polymerase B in a dinucleotide-primed reaction.酵母RNA聚合酶B在二核苷酸引发反应中的高效且选择性起始。
Nucleic Acids Res. 1981 Jan 10;9(1):31-45. doi: 10.1093/nar/9.1.31.
3
Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.利用lacZ融合来界定酿酒酵母中可诱导的双向GAL1 - GAL10启动子的调控元件。
J Virol. 1991 Feb;65(2):677-86. doi: 10.1128/JVI.65.2.677-686.1991.
Mol Cell Biol. 1984 Oct;4(10):1985-98. doi: 10.1128/mcb.4.10.1985-1998.1984.
4
DNA supercoiling and its effects on DNA structure and function.DNA超螺旋及其对DNA结构和功能的影响。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:85-91. doi: 10.1101/sqb.1983.047.01.011.
5
Size of the unwound region of DNA in Escherichia coli RNA polymerase and calf thymus RNA polymerase II ternary complexes.大肠杆菌RNA聚合酶和小牛胸腺RNA聚合酶II三元复合物中DNA解旋区域的大小。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:455-61. doi: 10.1101/sqb.1983.047.01.054.
6
On the action of the cyclic AMP-cyclic AMP receptor protein complex at the Escherichia coli lactose and galactose promoter regions.关于环磷酸腺苷-环磷酸腺苷受体蛋白复合物在大肠杆菌乳糖和半乳糖启动子区域的作用。
EMBO J. 1984 Jan;3(1):43-50. doi: 10.1002/j.1460-2075.1984.tb01759.x.
7
Stimulation of yeast RNA polymerase II transcription by critical values of supercoiling.
Biochemistry. 1984 Jan 3;23(1):69-73. doi: 10.1021/bi00296a011.
8
Topological modifications and template activation are induced in chimaeric plasmids by inserted sequences.插入序列可诱导嵌合质粒发生拓扑修饰和模板激活。
J Mol Biol. 1983 Mar 25;165(1):59-77. doi: 10.1016/s0022-2836(83)80242-3.
9
Hairpin-loop formation by inverted repeats in supercoiled DNA is a local and transmissible property.超螺旋DNA中反向重复序列形成发夹环是一种局部且可传递的特性。
Nucleic Acids Res. 1981 Mar 25;9(6):1271-89. doi: 10.1093/nar/9.6.1271.
10
E. coli RNA polymerase interacts homologously with two different promoters.大肠杆菌RNA聚合酶与两种不同的启动子进行同源性相互作用。
Cell. 1980 Jun;20(2):269-81. doi: 10.1016/0092-8674(80)90613-3.