School of Environmental Science & Optoelectronic Technology, University of Science & Technology of China, Hefei, Anhui, 230026, PR China.
Key Laboratory of High Magnetic Field & Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, PR China.
Nanomedicine (Lond). 2018 Aug;13(16):2067-2082. doi: 10.2217/nnm-2018-0036. Epub 2018 Sep 11.
To investigate the possible mechanisms of telomerase and telomere underlying the anticancer effects of silver nanoparticles (AgNPs).
MATERIALS & METHODS: 25nm polyvinylpyrrolidone-coated AgNPs were used. The telomerase activity and telomere function were evaluated. The anticancer effects of AgNPs were gauged with cell viability assay under different statement of telomerase and telomere.
RESULTS & CONCLUSION: AgNPs could inhibit telomerase activity and lead to telomere shortening and dysfunction. Overexpression of telomerase attenuated the anticancer activity of AgNPs, whereas downregulation of telomerase activity or dysfunction of the telomere enhanced the cytotoxicity of AgNPs in HeLa cells. Our findings provided strong evidence that the anticancer effects of AgNPs were mediated via interference with the telomerase/telomere.
探讨银纳米粒子（AgNPs）抗癌作用的端粒酶和端粒的可能机制。
使用 25nm 聚维酮包覆的 AgNPs。评估端粒酶活性和端粒功能。通过不同端粒酶和端粒状态下的细胞活力测定来评估 AgNPs 的抗癌作用。
AgNPs 可抑制端粒酶活性，导致端粒缩短和功能障碍。端粒酶的过表达减弱了 AgNPs 的抗癌活性，而端粒酶活性的下调或端粒功能障碍增强了 AgNPs 在 HeLa 细胞中的细胞毒性。我们的研究结果提供了强有力的证据，表明 AgNPs 的抗癌作用是通过干扰端粒酶/端粒介导的。
