Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea.
Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea.
Biomed Pharmacother. 2016 Dec;84:158-165. doi: 10.1016/j.biopha.2016.09.016. Epub 2016 Sep 16.
The pharmaceutical role of silver nanoparticles has been increased over the last decades, especially those synthesized through herbal medicinal plants, due to their variety of pharmacological importance. Panax ginseng Meyer (P. ginseng) has been widely used as a therapeutic herbal medicine for a long time in cancer treatment. In this study, the cytotoxic and oxidative effect of a novel silver nanoparticles synthesized from P. ginseng fresh leaves (P.g AgNPs) were evaluated in different human cancer cell lines. In addition, the effect of P.g AgNPs on cell migration, apoptosis and the determination of the mechanism involve was determinate by the use of A549 lung cancer cell line. It was found that P.g AgNPs treatment inhibited cell viability and induced oxidative stress in A549, MCF7 and HepG2 cancer cell lines. Likewise, P.g AgNPs treatment inhibited the epidermal growth factor (EGF)-enhanced migration, as well as decreased the mRNA levels and phosphorylation of EGF receptors in A549 cells. Moreover, P.g AgNPs modified the morphology of the cell nucleus and increase apoptosis percentage; this effect was linked to the stimulation of p38 MAPK/p53 pathway. Taken together, our results showed that P.g AgNPs exhibited anti-cancer activity in A549 and the regulation of EGFR/p38 MAPK/p53 pathway might be the possible mechanism of its anti-activity. Further experiments are suggested to determinate the mechanism by which P.g AgNPs induce cytotoxicity and ROS generation in MCF-7 and HepG2 cells.
在过去的几十年中，银纳米粒子的药物作用得到了增强，特别是那些通过草药植物合成的银纳米粒子，因为它们具有多种药理学重要性。人参（Panax ginseng Meyer）作为一种治疗草药，在癌症治疗中已经被广泛使用了很长时间。在这项研究中，评估了从新鲜人参叶合成的新型银纳米粒子（P.g AgNPs）对不同人类癌细胞系的细胞毒性和氧化作用。此外，通过使用 A549 肺癌细胞系，确定了 P.g AgNPs 对细胞迁移、凋亡的影响及其涉及的机制。结果发现，P.g AgNPs 处理抑制了 A549、MCF7 和 HepG2 癌细胞系的细胞活力并诱导了氧化应激。同样，P.g AgNPs 处理抑制了表皮生长因子（EGF）增强的迁移，并且降低了 A549 细胞中 EGF 受体的 mRNA 水平和磷酸化。此外，P.g AgNPs 改变了细胞核的形态并增加了凋亡百分比；这种作用与 p38 MAPK/p53 途径的刺激有关。总之，我们的结果表明，P.g AgNPs 在 A549 中表现出抗癌活性，并且调节 EGFR/p38 MAPK/p53 途径可能是其抗活性的可能机制。建议进一步实验以确定 P.g AgNPs 在 MCF-7 和 HepG2 细胞中诱导细胞毒性和 ROS 生成的机制。
