Department of Rehabilitation Medicine, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, China.
Department of Neurology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
Chin Med J (Engl). 2018 Sep 20;131(18):2172-2178. doi: 10.4103/0366-6999.240799.
The effectiveness of the combination of electroacupuncture (EA) and behavioral training (BT) for mid/advanced cerebral infarction (M/ACI) and related mechanisms remains unclear. This study aimed to investigate the combined effects on the learning-memory ability and event-related potential P300 in rats with M/ACI.
Eighty rats with M/ACI were divided into Group Model (M), Group EA, Group BT, and Group EA-BT (n = 20) according to the random number with five healthy rats in Group Control (CON). On the 6 week after modeling, EA, BT, and EA-BT were given to Group EA, Group BT, and Group EA-BT, respectively, whereas Group M and Group CON were not given any intervention. Y-maze test and P300 were recorded before and after the intervention.
After intervention, the P300 latency was lower and the amplitude was higher in the Group EA-BT, Group EA, and Group BT than before (for latency, t = -7.638, -4.334, and -5.916; for amplitude, t = 8.125, 3.846, and 5.238; P < 0.01), with Group EA-BT superior to Group EA (for latency, t = -3.708; for amplitude, t = 3.653; P < 0.01) and Group BT (for latency, t = -2.067; for amplitude, t = 2.816; P < 0.05), with no significant difference between Group BT and EA (for latency, t = -1.439; for amplitude, t = 1.075; P > 0.05). While the performances of Y-maze tests in the Group EA-BT, Group EA, and Group BT were all better than before (t = 10.359, 4.520, and 7.791, P < 0.01), with Group EA-BT better than Group EA (t = 5.627, P < 0.01) and Group BT (t = 2.913, P < 0.01) respectively, and Group BT better than Group EA (t = 2.912, P < 0.01).
EA or BT can affect P300 in rats with M/ACI, and the combination of these two methods can significantly improve the learning-memory ability.
电针（EA）联合行为训练（BT）对中/重度脑梗死（M/ACI）的有效性及其相关机制尚不清楚。本研究旨在探讨 EA 和 BT 联合应用对 M/ACI 大鼠学习记忆能力和事件相关电位 P300 的影响。
80 只 M/ACI 大鼠按随机数字表法分为模型组（M）、电针组（EA）、行为训练组（BT）和 EA+BT 联合组（EA-BT），每组 20 只，同时设 5 只正常大鼠为对照组（CON）。建模 6 周后，EA、BT 和 EA-BT 组分别给予 EA、BT 和 EA-BT 干预，M 组和 CON 组不予任何干预。于干预前后行 Y 迷宫测试和 P300 检测。
干预后，EA-BT 组、EA 组和 BT 组大鼠 P300 潜伏期较干预前降低，波幅较干预前升高（潜伏期：t=-7.638、-4.334 和-5.916；波幅：t=8.125、3.846 和 5.238；P<0.01），其中 EA-BT 组优于 EA 组（潜伏期：t=-3.708；波幅：t=3.653；P<0.01）和 BT 组（潜伏期：t=-2.067；波幅：t=2.816；P<0.05），BT 组与 EA 组比较差异无统计学意义（潜伏期：t=-1.439；波幅：t=1.075；P>0.05）。同时，EA-BT 组、EA 组和 BT 组大鼠 Y 迷宫测试成绩均优于干预前（t=10.359、4.520 和 7.791，P<0.01），其中 EA-BT 组优于 EA 组（t=5.627，P<0.01）和 BT 组（t=2.913，P<0.01），BT 组优于 EA 组（t=2.912，P<0.01）。
EA 或 BT 均可影响 M/ACI 大鼠 P300，且两法联合应用可显著提高大鼠学习记忆能力。
