Department of Biophysics, Faculty of Medicine, Akdeniz University, Arapsuyu, 07070, Antalya, Turkey.
Department of Biophysics, Faculty of Medicine, Akdeniz University, Arapsuyu, 07070, Antalya, Turkey.
Neurotoxicology. 2017 Sep;62:64-74. doi: 10.1016/j.neuro.2017.05.003. Epub 2017 May 10.
Rosmarinic acid (RA), which has multiple bioactive properties, might be a useful agent for protecting central nervous system against age related alterations. In this context, the purpose of the present study was to investigate possible protective effects of RA on mismatch negativity (MMN) component of auditory event-related potentials (AERPs) as an indicator of auditory discrimination and echoic memory in the ovariectomized (OVX) rats injected with d-galactose combined with neurochemical and histological analyses. Ninety female Wistar rats were randomly divided into six groups: sham control (S); RA-treated (R); OVX (O); OVX+RA-treated (OR); OVX+d-galactose-treated (OD); OVX+d-galactose+RA-treated (ODR). Eight weeks later, MMN responses were recorded using the oddball condition. An amplitude reduction of some components of AERPs was observed due to ovariectomy with or without d-galactose administiration and these reduction patterns were diverse for different electrode locations. MMN amplitudes were significantly lower over temporal and right frontal locations in the O and OD groups versus the S and R groups, which was accompanied by increased thiobarbituric acid reactive substances (TBARS) and hydroxy-2-nonenal (4-HNE) levels. RA treatment significantly increased AERP/MMN amplitudes and lowered the TBARS/4-HNE levels in the OR and ODR groups versus the O and OD groups, respectively. Our findings support the potential benefit of RA in the prevention of auditory distortion related to the estrogen deficiency and d-galactose administration at least partly by antioxidant actions.
迷迭香酸（RA）具有多种生物活性，可能是一种有用的药物，可保护中枢神经系统免受与年龄相关的改变。在这方面，本研究的目的是研究 RA 对听觉事件相关电位（AERPs）中不匹配负波（MMN）成分的可能保护作用，作为听觉辨别和回声记忆的指标，在注射 D-半乳糖的去卵巢（OVX）大鼠中进行，结合神经化学和组织学分析。90 只雌性 Wistar 大鼠随机分为六组：假手术对照（S）；RA 处理（R）；OVX（O）；OVX+RA 处理（OR）；OVX+D-半乳糖处理（OD）；OVX+D-半乳糖+RA 处理（ODR）。8 周后，使用偶极子条件记录 MMN 反应。由于卵巢切除术，无论是否给予 D-半乳糖，AERPs 的某些成分的振幅都降低了，并且这些降低模式因不同的电极位置而异。与 S 和 R 组相比，O 和 OD 组的 MMN 振幅在颞部和右额部明显降低，这伴随着硫代巴比妥酸反应物质（TBARS）和 4-羟基-2-壬烯醛（4-HNE）水平的增加。RA 处理在 OR 和 ODR 组中显著增加了 AERP/MMN 振幅，并降低了 TBARS/4-HNE 水平，与 O 和 OD 组相比。我们的研究结果支持 RA 在预防与雌激素缺乏和 D-半乳糖给药相关的听觉失真方面的潜在益处，至少部分是通过抗氧化作用。
