Kiran Shashi, Schumann Peter, Busse Hans-Jürgen, Spröer Cathrin, Rana Aditi, Pal Mohinder, Korpole Suresh, Tewari Rupinder, Gulati Arvind
1Microbial Prospection Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur (H.P.)-176 061, India.
2Department of Microbial Biotechnology, Panjab University, Chandigarh - 160 014, India.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3416-3423. doi: 10.1099/ijsem.0.002997. Epub 2018 Sep 11.
The novel strain IHBB 11108 was a psychrotolerant and alkaliphilic bacterium isolated from the subsurface water of Chandra Tal Lake in the Lahaul-Spiti valley located in the Indian trans-Himalayas. Cells were Gram-stain-positive, aerobic, non-motile, catalase-positive and oxidase-negative. The strain grew at 5-37 °C (optimum 28 °C), pH 5.0-12.0 (optimum pH 7.0) and with up to 8 % NaCl (optimum 1 %). The 16S rRNA gene sequence analysis revealed the highest relatedness of strain IHBB 11108 with Psychromicrobium silvestre DSM 102047 (97.5 %), Arthrobacter russicus DSM 14555 (97.4 %) and Renibacterium salmoninarum ATCC 33209 (97.4 %). The strain contained a quinone system with 57.2 % MK-9(H2), 39.1 % MK-10(H2), 3.0 % MK-8(H2) and 0.7 % MK-7(H2). The polar lipids detected were diphosphatidylglycerol, dimannosylglyceride, phosphatidylinositol, phosphatidylglycerol, monogalactosyldiacylglycerol, one unidentified glycolipid and four unidentified lipids. The cell-wall peptidoglycan structure type was A3α l-Lys-l-Thr-l-Ala with substitution of the α-carboxyl group of d-Glu by alanine amide. Anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were the predominant fatty acids. The genomic DNA G+C content was 59.0 mol%. The DNA-DNA relatedness of strain IHBB 11108 was 46.7±2.2, 43.1±2.5 and 19.1±2.4 % with P. silvestre DSM 102047, A. russicus DSM 14555 and R. salmoninarum ATCC 33209, respectively. On the basis of the results of the phenotypic, chemotaxonomic and phylogenetic analyses, IHBB 11108 is considered to represent a novel species of the genus Psychromicrobium for which the name Psychromicrobium lacuslunae sp. nov. is proposed. The type strain is IHBB 11108 (=MTCC 12460=MCC 2780=JCM 31143=KACC 19070).
新型菌株IHBB 11108是一种耐冷嗜碱细菌，从位于印度跨喜马拉雅地区拉胡尔-斯皮提山谷的钱德拉塔尔湖地下水中分离得到。细胞革兰氏染色阳性，好氧，不运动，过氧化氢酶阳性，氧化酶阴性。该菌株在5-37 °C（最适温度28 °C）、pH 5.0-12.0（最适pH 7.0）以及高达8 % NaCl（最适1 %）的条件下生长。16S rRNA基因序列分析表明，菌株IHBB 11108与西尔维斯特嗜冷微菌DSM 102047（97.5 %）、俄罗斯节杆菌DSM 14555（97.4 %）和鲑鱼肾杆菌ATCC 33209（97.4 %）的亲缘关系最高。该菌株含有一个醌系统，其中MK-9(H2)占57.2 %，MK-10(H2)占39.1 %，MK-8(H2)占3.0 %，MK-7(H2)占0.7 %。检测到的极性脂质有双磷脂酰甘油、二甘露糖基甘油酯、磷脂酰肌醇、磷脂酰甘油、单半乳糖基二酰基甘油、一种未鉴定的糖脂和四种未鉴定的脂质。细胞壁肽聚糖结构类型为A3α l-Lys-l-Thr-l-Ala型，d-Glu的α-羧基被丙氨酰胺取代。anteiso-C15 : 0、iso-C16 : 0和anteiso-C17 : 0是主要脂肪酸。基因组DNA的G+C含量为59.0 mol%。菌株IHBB 11108与西尔维斯特嗜冷微菌DSM 102047、俄罗斯节杆菌DSM 14555和鲑鱼肾杆菌ATCC 33209的DNA-DNA相关性分别为46.7±2.2、43.1±2.5和19.1±2.4 %。基于表型、化学分类和系统发育分析结果，IHBB 11108被认为代表嗜冷微菌属的一个新物种，为此提出新名称嗜冷微菌湖新月菌（Psychromicrobium lacuslunae sp. nov.）。模式菌株为IHBB 11108（=MTCC 12460=MCC 2780=JCM 31143=KACC 19070）。
