State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences,, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
National Culture Collection of Pakistan (NCCP), Bio-Resources Conservation Institute (BCI), National Agricultural Research Centre (NARC), Park Road, Islamabad, 45500, Pakistan.
Arch Microbiol. 2021 May;203(4):1717-1729. doi: 10.1007/s00203-020-02176-4. Epub 2021 Jan 18.
A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664, was isolated from Cholistan desert, Pakistan. Cells of strain NCCP-1664 were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%). Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664 belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa. DNA-DNA relatedness values of strain NCCP-1664 with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively. DNA G + C content of strain NCCP-1664 was 70.0 mol%. Chemotaxonomic data of strain NCCP-1664 showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H) (67%), MK-8(H) (32%) and MK-7(H) (1%), major fatty acids as anteiso -C (51.2%), anteiso-C (9.6%) and Cω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids. The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp. nov. is proposed with the type strain NCCP-1664 (= DSM 29936 = KCTC 39549).
从巴基斯坦的 Cholistan 沙漠中分离到一株革兰氏染色阳性、非孢子形成、非色素和非运动的细菌，命名为 NCCP-1664 菌株。NCCP-1664 菌株为严格需氧、过氧化氢酶阳性、氧化酶阴性，具有杆状到球菌的生长周期，可在 pH6.0-9.0（最佳 pH7-8）、28-45°C（最佳 37°C）下生长，并能耐受 0-16%的 NaCl（最佳 2%）。基于 16S rRNA 基因序列的系统发育分析表明，NCCP-1664 菌株属于微球菌科，与节杆菌属的成员关系最为密切，与银杏节杆菌（98.9%）、盐单胞菌（97.7%）和米曲霉（97.1%）的序列相似度最高，与其他相关分类群的相似度低于 97%。与上述模式菌株的 DNA-DNA 同源性值低于 54%，而与米曲霉的数字 DDH 和平均核苷酸同一性（ANI）值分别为 20.9%和 74.3%。NCCP-1664 菌株的 DNA G+C 含量为 70.0mol%。NCCP-1664 菌株的化学生物分类数据显示其肽聚糖类型为 A3α L-Lys-L-Ala；类脂醌为 MK-9(H)（67%）、MK-8(H)（32%）和 MK-7(H)（1%）；主要脂肪酸为 anteiso-C（51.2%）、anteiso-C（9.6%）和 Cω9c（6.9%）；极性脂类谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、双半乳糖基二酰基甘油、少量单半乳糖基二酰基甘油、三甘露糖基二酰基甘油和三种未鉴定的脂类组成。系统发育分析以及化学生物分类数据、生理生化特性表明，它是一个新属的代表，因此命名为 Zafaria cholistanensis 属，nov. 种。该属的模式菌株为 NCCP-1664 菌株（=DSM 29936=KCTC 39549）。
