Bresler V M, Nikiforov A A
Tsitologiia. 1977;19(1):82-9.
By direct microfluorimetry, the kinetic characteristics of active transport system for organic acid were studied. A removal of sodium from the incubation medium is shown to affect the affinity between a carrier and acids (KS), and the rate of translocation of the transporting complex (which includes a carrier and a substrate) across the plasma membrane. The data obtained are in good accordance with the concept of Na+-dependent transport.
通过直接显微荧光测定法,研究了有机酸主动转运系统的动力学特性。结果表明,从孵育培养基中去除钠会影响载体与酸之间的亲和力(KS),以及转运复合物(包括载体和底物)跨质膜的转运速率。所得数据与钠依赖性转运的概念高度相符。