Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Chennai 600062, India.
Department of Biotechnology, St. Joseph's College of Engineering, Chennai 600119, India.
Bioresour Technol. 2018 Dec;269:393-399. doi: 10.1016/j.biortech.2018.09.002. Epub 2018 Sep 4.
In the present investigation, the effects of crude glycerol concentration, spore inoculum concentration, yeast extract concentration and shaking frequency on seed morphology of Aspergillus niger PJR1 on malic acid production were investigated and dispersed fungal mycelium with higher biomass (20.25 ± 0.91 g/L) was obtained when A. niger PJR1 grow on crude glycerol. Dry cell weight under dispersed fermentation was 21.28% higher than usual pellet fermentation. The optimal crude glycerol, nitrogen source and nitrogen source concentration were found to be 160 g/L, yeast extract and 1.5 g/L, respectively. Batch fermentation in a shake flask culture containing 160 g/L crude glycerol resulted in the yield of malic acid 83.23 ± 1.86 g/L, after 192 h at 25 °C. Results revealed that morphological control of A. niger is an efficient method for increased malic acid production when crude glycerol derived from biodiesel production is used as feedstock.
在本研究中，考察了粗甘油浓度、孢子接种浓度、酵母提取物浓度和摇床频率对黑曲霉 PJR1 在产苹果酸过程中种子形态的影响。结果表明，当黑曲霉 PJR1 在粗甘油上生长时，获得了具有更高生物量（20.25±0.91 g/L）的分散真菌菌丝体。与常规颗粒发酵相比，分散发酵的干细胞重量高 21.28%。发现最佳粗甘油、氮源和氮源浓度分别为 160 g/L、酵母提取物和 1.5 g/L。在含有 160 g/L 粗甘油的摇瓶培养物中进行分批发酵，在 25°C 下培养 192 h 后，苹果酸的产量为 83.23±1.86 g/L。结果表明，当以生物柴油生产中产生的粗甘油为原料时，控制黑曲霉的形态是提高苹果酸产量的有效方法。
