Salimi Z, Wolverson M K, Herbold D R, Vas W
Radiology. 1986 Oct;161(1):227-31. doi: 10.1148/radiology.161.1.3020608.
We designed an experimental model using a new method of freezing to study the pathogenesis and treatment of frostbite. Frostbite was simulated in a manner that closely resembles that which occurs in a natural environment. We used a radionuclide imaging technique to monitor the evolution and extent of tissue damage relative to temperature, rate of freezing, and controlled rewarming. Characteristic sequential changes were demonstrated on sequential nuclear scans. Nonperfusion, followed by perfusion, and finally again by nonperfusion occurred in all areas in which necrosis developed. The reappearance of nonperfusion corresponded to vascular injury and thrombosis evidenced at pathologic examination. We determined that lack of tissue perfusion corresponded to tissue injury. We believe that our experimental model provides an effective means of evaluating potential therapeutic regimens.
我们采用一种新的冷冻方法设计了一个实验模型，以研究冻伤的发病机制和治疗方法。冻伤的模拟方式与自然环境中发生的情况极为相似。我们使用放射性核素成像技术来监测相对于温度、冷冻速率和控制性复温的组织损伤的演变及范围。在连续的核扫描中显示出特征性的序列变化。在所有发生坏死的区域，先是出现无灌注，接着是灌注，最后又再次出现无灌注。无灌注的再次出现与病理检查中证实的血管损伤和血栓形成相对应。我们确定组织灌注的缺乏与组织损伤相对应。我们认为我们的实验模型为评估潜在的治疗方案提供了一种有效的手段。
