National Center for Transgenic Research in Plants, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Sci Rep. 2018 Sep 11;8(1):13601. doi: 10.1038/s41598-018-31703-z.
Aluminium (Al) toxicity restrains water and nutrient uptake and is toxic to plant roots, ultimately inhibiting crop production. Here, we isolated and characterized a soybean glycine-rich protein-like gene (GmGRPL) that is mainly expressed in the root and that is regulated by Al treatment. Overexpression of GmGRPL can alleviate Al-induced root growth inhibition in Arabidopsis. The levels of IAA and ethylene in GmGRPL-overexpressing hairy roots were lower than those in control and RNA interference-exposed GmGRPL hairy roots with or without Al stress, which were mainly regulated by TAA1 and ACO, respectively. In transgenic soybean hairy roots, the MDA, HO and Ocontent in GmGRPL-overexpressing hairy roots were less than that in control and RNA interference-exposed GmGRPL hairy roots under Al stress. In addition, IAA and ACC can enhance the expression level of the GmGRPL promoter with or without Al stress. These results indicated that GmGRPL can alleviate Al-induced root growth inhibition by regulating the level of IAA and ethylene and improving antioxidant activity.
铝（Al）毒性会抑制水和养分的吸收，对植物根系有毒害作用，最终会抑制作物的生产。在这里，我们分离并鉴定了一个大豆富含甘氨酸的蛋白样基因（GmGRPL），该基因主要在根中表达，并受 Al 处理的调节。过表达 GmGRPL 可以减轻拟南芥中 Al 诱导的根生长抑制。在 GmGRPL 过表达毛状根中，IAA 和乙烯的水平低于对照和 RNA 干扰暴露的 GmGRPL 毛状根，无论是否有 Al 胁迫，这些主要受 TAA1 和 ACO 的调节。在转基因大豆毛状根中，在 Al 胁迫下，GmGRPL 过表达毛状根中的 MDA、HO 和 O 含量均低于对照和 RNA 干扰暴露的 GmGRPL 毛状根。此外，IAA 和 ACC 可以增强 GmGRPL 启动子的表达水平，无论是否有 Al 胁迫。这些结果表明，GmGRPL 通过调节 IAA 和乙烯的水平以及提高抗氧化活性来减轻 Al 诱导的根生长抑制。
