生物基质对基于靶向质谱的炎症蛋白生物标志物定量的影响
Impact of biological matrix on inflammatory protein biomarker quantification based on targeted mass spectrometry.
作者信息
Incamps Anne, Saez-Boiteau Celia, Tiede Svenja Lena, Rebillard Pauline, Schulte Janin, Vialaret Jérôme, Beinrucker Andre, Lehmann Sylvain, Hirtz Christophe
机构信息
Thermo Fisher Scientific Cezanne SAS, Clinical Diagnostics Division, 280 allée Graham Bell, 30000 Nîmes, France.
Montpellier University, LBPC/PPC - IRMB, CHU de Montpellier, INSERM, 80 rue Augustin Fliche, Montpellier, France.
出版信息
Bioanalysis. 2018 Sep 1;10(17):1383-1399. doi: 10.4155/bio-2018-0149. Epub 2018 Sep 12.
BACKGROUND
Serum and plasma are widely used matrices in biological and clinical studies. To improve reliability and consistency of markers quantification, the influence of these matrices on proteins was evaluated by targeted mass spectrometry.
RESULTS
65 proteins were quantified in matched blood samples collected in serum, ethylenediaminetetraacetic acid and heparin plasma tubes from 40 healthy and 10 pathological individuals. Only 52% of the proteins were not impacted by any of the biological matrices tested, and the effects on quantification of proteins affected was matrix and protein dependent.
CONCLUSION
Matrix comparisons using mass spectrometry is therefore recommended to assess the relevance of using surrogate matrix, performing biomarker discovery study or evaluating the clinical use of biomarkers in large clinical cohorts.
背景
血清和血浆是生物学和临床研究中广泛使用的基质。为提高标志物定量的可靠性和一致性，通过靶向质谱法评估了这些基质对蛋白质的影响。
结果
在从40名健康个体和10名病理个体采集的血清、乙二胺四乙酸和肝素血浆管中收集的匹配血液样本中，对65种蛋白质进行了定量。只有52%的蛋白质不受任何测试生物基质的影响，并且对受影响蛋白质定量的影响取决于基质和蛋白质。
结论
因此，建议使用质谱法进行基质比较，以评估使用替代基质、进行生物标志物发现研究或评估生物标志物在大型临床队列中的临床应用的相关性。
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