Perlmutter Cancer Center, NYU School of Medicine, New York, NY, USA.
Columbia Center of Translational Immunology, Department of Medicine, Columbia University Medical Center, New York, NY, USA.
Eur J Immunol. 2018 Nov;48(11):1915-1917. doi: 10.1002/eji.201847655. Epub 2018 Oct 3.
Exosomes are cell-derived vesicles that have been implicated in the pathogenesis of many inflammatory diseases. More specifically, it has been shown that T cell-derived exosomes can induce immunological responses; however, little is known about the mechanism and the molecular content of these vesicles. Here, we used a proteomic approach to characterize human T cell-derived exosomes. We found that specific proteins of the RAS signaling pathway were enriched in exosomes derived from activated T cells, and that these vesicles induced ERK phosphorylation in recipient immune cells. Our findings support a mechanistic role of exosomes in cellular activation, and further studies should consider exosomes as a biomarker for inflammatory diseases.
外泌体是细胞来源的囊泡，已被牵连到许多炎症性疾病的发病机制中。更具体地说，已经表明 T 细胞来源的外泌体可以诱导免疫反应；然而，对于这些囊泡的机制和分子内容知之甚少。在这里，我们使用蛋白质组学方法来描述人 T 细胞来源的外泌体。我们发现，激活的 T 细胞来源的外泌体中富含 RAS 信号通路的特定蛋白质，并且这些囊泡诱导受体免疫细胞中的 ERK 磷酸化。我们的发现支持外泌体在细胞激活中的机制作用，进一步的研究应将外泌体视为炎症性疾病的生物标志物。
