Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health , Southeast University , Nanjing 210009 , China.
Suzhou Municipal Center for Disease Prevention and Control , Suzhou 215004 , China.
J Agric Food Chem. 2018 Oct 10;66(40):10588-10597. doi: 10.1021/acs.jafc.8b03082. Epub 2018 Sep 26.
In this work, male rats were exposed to multiple phthalate esters (MIXPs) in a long-term low-dose model for the early evaluation of reproductive toxicity. An ananlysis method with better sensitivity, accuracy and precision was established to determine the five sex hormones (androstenedione, testosterone, dehydroepiandrosterone, dihydrotestosterone, and estrone) in collected urine samples. The results showed that all the analytes in the MIXPs treated group changed in a time-dependent manner. Specifically, estrone significantly decreased from the 30th day and the other four changed from the 30th day and then significantly increased on the 60th day, while no obvious changes were found in the control group. Therefore, a possible way was provided for the early evaluation of male reproductive toxicity induced by Phthalate esters (PEs) . The reliability of judgment was improved by observing the changes of five target hormones simultaneously. Furthermore, good compliance was predicted for the practical application due to the noninvasive and convenient urine sample collection.
在这项工作中，雄性大鼠在长期低剂量模型中暴露于多种邻苯二甲酸酯（MIXPs）中，以早期评估生殖毒性。建立了一种灵敏度、准确性和精密度更好的分析方法，以确定收集的尿液样本中的五种性激素（雄烯二酮、睾酮、脱氢表雄酮、双氢睾酮和雌酮）。结果表明，MIXPs 处理组中的所有分析物均呈时间依赖性变化。具体来说，雌酮从第 30 天开始显著下降，其他四种从第 30 天开始然后在第 60 天显著增加，而对照组没有明显变化。因此，为邻苯二甲酸酯（PEs）引起的男性生殖毒性的早期评估提供了一种可能的方法。通过同时观察五种目标激素的变化，提高了判断的可靠性。此外，由于尿液样本采集非侵入性且方便，因此预计具有良好的实用性。
Zotero 插件