用于基因传递的基于层层组装的聚乙烯亚胺载体与上转换发光标记物。
Layer-by-layer assembled PEI-based vector with the upconversion luminescence marker for gene delivery.
机构信息
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, School of Chemistry and Materials Science, Nanjing Normal University, Wenyuan Road 1, Nanjing, 210046, China.
Shanghai Jahwa United Co., Ltd, Baoding Road 527, Shanghai, 200082, China.
出版信息
Biochem Biophys Res Commun. 2018 Sep 18;503(4):2504-2509. doi: 10.1016/j.bbrc.2018.07.007. Epub 2018 Jul 5.
The upconversion luminescence (UCL) marker based on upconversion nanoparticles (UCNPs) shows unique advantages over traditional fluorescence markers, such as enhanced tissue penetration, better photostability, and less autofluorescence. Herein, we constructed a new UCL gene-delivery nonviral vector via layer-by-layer self-assembly of poly(ethylene imine) (PEI) with UCNPs. To reduce the cytotoxicity of PEI, citric acid (CA) was introduced for aqueous modification, and PEI assembly was introduced on the UCNP surface. Our data show that the nonviral vector for UCL gene-delivery demonstrates excellent photostability, low toxicity, and good stability under physiological or serum conditions and can strongly bind to DNA. Moreover, this UCL PEI-based vector could serve as a promising fluorescent gene-delivery carrier for theranostic applications.
上转换发光（UCL）标记物基于上转换纳米粒子（UCNPs），与传统荧光标记物相比具有独特的优势，例如增强组织穿透性、更好的光稳定性和更少的自发荧光。在此，我们通过逐层自组装聚（亚乙基亚胺）（PEI）与 UCNPs 构建了一种新型 UCL 基因传递非病毒载体。为了降低 PEI 的细胞毒性，引入柠檬酸（CA）进行水相修饰，并在 UCNP 表面引入 PEI 组装。我们的数据表明，用于 UCL 基因传递的非病毒载体具有优异的光稳定性、低毒性和在生理或血清条件下的良好稳定性，并且可以与 DNA 强烈结合。此外，这种基于 UCL 的 PEI 载体可以作为一种有前途的荧光基因传递载体，用于治疗应用。
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