Department of Oncology Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China (mainland).
Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China (mainland).
Med Sci Monit. 2018 Sep 13;24:6414-6421. doi: 10.12659/MSM.909443.
BACKGROUND Gastrointestinal stromal tumor (GIST) is an uncommon visceral sarcoma that arises predominantly in the gastrointestinal tract. Since GISTs are encountered infrequently and inflexible to traditional therapy, the aim of the present study was to explore the correlation of B-cell-specific Moloney murine leukemia virus insertion site 1 (BMI-1) mRNA and BMI-1 protein levels with the clinicopathological characteristics and prognosis significance of GISTs. MATERIAL AND METHODS GIST tissues and normal tissues were collected from 156 patients who had undergone surgical treatment. RT-qPCR and immunohistochemistry were used to measure the BMI-1 mRNA and protein levels in GIST tissues and normal tissues. Univariate survival analysis was used for determination of the factors that affect prognosis of GIST patients. Cox proportional hazards model was plotted to determine the independent risk factors for prognosis of GIST patients. RESULTS The BMI-1 mRNA and protein levels in GIST tissues were higher than those in normal tissues. BMI-1 mRNA and positive protein levels were correlated with the National Institutes of Health (NIH) risk grade, tumor diameter and infiltration, and metastasis. There was a short survival period for the patients with a positive protein level and a high mRNA level of BMI-1. The site of primary tumor, tumor diameter, NIH risk grade, infiltration, and metastasis, as well as BMI-1 mRNA and protein levels were independent risk factors for prognosis of GIST patients. CONCLUSIONS Taken together, these findings suggest there might be a relationship between BMI-1 mRNA and protein levels, and clinicopathological characteristics, including NIH risk grade, tumor size as well as infiltration and metastasis, of GIST patients. In addition, BMI-1 mRNA and protein levels were identified as independent risk factors for prognosis of GIST patients.
胃肠道间质瘤（GIST）是一种罕见的内脏肉瘤，主要发生在胃肠道。由于 GIST 很少见，对传统治疗方法不敏感，本研究旨在探讨 B 细胞特异性 Moloney 鼠白血病病毒插入位点 1（BMI-1）mRNA 和 BMI-1 蛋白水平与 GIST 临床病理特征和预后意义的相关性。
收集 156 例接受手术治疗的 GIST 患者的 GIST 组织和正常组织。采用 RT-qPCR 和免疫组织化学法检测 GIST 组织和正常组织中 BMI-1 mRNA 和蛋白水平。采用单因素生存分析确定影响 GIST 患者预后的因素。采用 Cox 比例风险模型绘制图确定 GIST 患者预后的独立危险因素。
GIST 组织中的 BMI-1 mRNA 和蛋白水平均高于正常组织。BMI-1 mRNA 和阳性蛋白水平与美国国立卫生研究院（NIH）风险分级、肿瘤直径和浸润、转移有关。BMI-1 蛋白阳性和高 mRNA 水平的患者生存期较短。原发肿瘤部位、肿瘤直径、NIH 风险分级、浸润和转移以及 BMI-1 mRNA 和蛋白水平是 GIST 患者预后的独立危险因素。
综上所述，BMI-1 mRNA 和蛋白水平与 GIST 患者的临床病理特征，包括 NIH 风险分级、肿瘤大小以及浸润和转移有关。此外，BMI-1 mRNA 和蛋白水平是 GIST 患者预后的独立危险因素。
