SLITRK3表达与胃肠道间质瘤风险分级及预后的相关性
SLITRK3 expression correlation to gastrointestinal stromal tumor risk rating and prognosis.
作者信息
Wang Chao-Jie, Zhang Zi-Zhen, Xu Jia, Wang Ming, Zhao Wen-Yi, Tu Lin, Zhuang Chun, Liu Qiang, Shen Yan-Yin, Cao Hui, Zhang Zhi-Gang
机构信息
Chao-Jie Wang, Zi-Zhen Zhang, Jia Xu, Ming Wang, Wen-Yi Zhao, Lin Tu, Chun Zhuang, Hui Cao, Department of Gastrointestinal Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
出版信息
World J Gastroenterol. 2015 Jul 21;21(27):8398-407. doi: 10.3748/wjg.v21.i27.8398.
AIM
To assess the influence of SLIT and NTRK-like family member 3 (SLITRK3) on the prognosis of gastrointestinal stromal tumor (GIST) and determine whether SLITRK3 can help improve current risk stratification systems.
METHODS
We hypothesized that SLITRK3 could be used as a prognostic molecular biomarker for GIST. 35 fresh tumor samples and 417 paraffin-embedded specimens from GIST patients were utilized. SLITRK3 mRNA expression in GIST tumor tissue was detected by real-time polymerase chain reaction, and SLITRK3 protein levels were estimated by immunohistochemistry. The correlation of SLITRK3 expression with various tumor clinicopathological characteristics and follow-up data were analyzed.
RESULTS
GIST tumors had high expression of SLITRK3 compared with adjacent normal tissues and the expression level gradually increased with risk grade. SLITRK3 protein expression was closely associated with gastrointestinal bleeding, tumor site, tumor size, mitotic index, and National Institutes of Health (NIH) classification. Survival analysis showed that SLITRK3 expression was closely correlated with overall survival and disease-free survival of GIST patients. Multivariate analysis also identified SLITRK3 expression, mitotic index, and NIH stage as significant risk factors of GIST recurrence.
CONCLUSION
SLITRK3 expression is a highly significant predictor of GIST recurrence and metastasis. Combinations of SLITRK3 and NIH stage have strong predictive and prognostic value, and are feasible markers for clinical practice in gastrointestinal stromal tumor.
目的
评估类Slit和NTRK样家族成员3（SLITRK3）对胃肠道间质瘤（GIST）预后的影响，并确定SLITRK3是否有助于改进当前的风险分层系统。
方法
我们假设SLITRK3可作为GIST的预后分子生物标志物。使用了来自GIST患者的35个新鲜肿瘤样本和417个石蜡包埋标本。通过实时聚合酶链反应检测GIST肿瘤组织中SLITRK3 mRNA的表达，并通过免疫组织化学评估SLITRK3蛋白水平。分析了SLITRK3表达与各种肿瘤临床病理特征及随访数据的相关性。
结果
与相邻正常组织相比，GIST肿瘤中SLITRK3表达较高，且表达水平随风险等级逐渐升高。SLITRK3蛋白表达与胃肠道出血、肿瘤部位、肿瘤大小、有丝分裂指数和美国国立卫生研究院（NIH）分类密切相关。生存分析表明，SLITRK3表达与GIST患者的总生存期和无病生存期密切相关。多变量分析还确定SLITRK3表达、有丝分裂指数和NIH分期为GIST复发的重要危险因素。
结论
SLITRK3表达是GIST复发和转移的高度显著预测指标。SLITRK3与NIH分期的联合具有很强的预测和预后价值，是胃肠道间质瘤临床实践中可行的标志物。
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