Famuyiwa O O, Akanji A O, Bella A F
Afr J Med Med Sci. 1986 Mar-Jun;15(1-2):49-53.
Haemoglobin A1 (HbA1) levels were measured in forty-five diabetic Nigerians and thirteen non-diabetic controls using disposable microcolumn kits based on affinity chromatography. The effect on HbA1 results of storage of whole blood samples for 1 week at room temperature and in a refrigerator was also evaluated. The mean +/- s.d. of HbA1 levels in diabetic patients and controls were 12.1 +/- 5.6% and 5.2 +/- 0.90% respectively. The difference was highly significant, P less than 0.001. Newly diagnosed diabetic patients who had not been on any treatment had significantly higher mean value of HbA1, 14.4 +/- 5.0%, than old patients already on treatment, 9.5 +/- 2.9%, P less than 0.01. In a group of nine patients, the mean +/- s.d. of baseline HbA1 value was 10.3 +/- 4.2% and after 1 week storage of different portions of the samples at room temperature and in a refrigerator, the mean +/- s.d. respectively were 9.8 +/- 3.6% and 11.0 +/- 4.2%. There was thus a slight decrease on storage at room temperature and a slight increase during refrigeration but the observed differences were not statistically significant. It is concluded that the glyc-affinity micro-column chromatography is a satisfactory and potentially useful method for measuring HbA1 levels in tropical developing countries. The method was easy to use and the assays could be run under average ambient room temperature without recourse to use of an air-conditioned room or a special chromatography chamber.
采用基于亲和色谱的一次性微柱试剂盒，对45名尼日利亚糖尿病患者和13名非糖尿病对照者进行了血红蛋白A1（HbA1）水平测定。还评估了全血样本在室温及冰箱中储存1周对HbA1检测结果的影响。糖尿病患者和对照者HbA1水平的均值±标准差分别为12.1±5.6%和5.2±0.90%。差异非常显著，P<0.001。未接受任何治疗的新诊断糖尿病患者的HbA1均值显著高于已接受治疗的老年患者，分别为14.4±5.0%和9.5±2.9%，P<0.01。在一组9名患者中，基线HbA1值的均值±标准差为10.3±4.2%，样本不同部分在室温及冰箱中储存1周后，均值±标准差分别为9.8±3.6%和11.0±4.2%。因此，室温储存时有轻微下降，冷藏时有轻微上升，但观察到的差异无统计学意义。结论是，糖亲和微柱色谱法是热带发展中国家测定HbA1水平的一种令人满意且可能有用的方法。该方法易于使用，检测可在平均室温下进行，无需使用空调房间或特殊色谱室。
