Takahashi Tetsuaki, Krulwich Terry A, Ito Masahiro
Graduate School of Life Sciences, Toyo University, Gunma, Japan.
Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
Front Microbiol. 2018 Aug 28;9:1994. doi: 10.3389/fmicb.2018.01994. eCollection 2018.
A monocistronic small protein, BpOF4_01690, was annotated in alkaliphilic OF4. It comprises 59 amino acids and is hydrophobic. Importantly, homologs of this protein were identified only in alkaliphiles. In this study, a mutant with a BpOF4_01690 gene deletion (designated Δ01690) exhibited weaker growth than that of the wild type in both malate-based defined and glucose-based defined media under low-sodium conditions at pH 10.5. Additionally, the enzymatic activity of the respiratory chain of Δ01690 was much lower than that of the wild type. These phenotypes were similar to those of a deletion mutant and an deletion mutant. Therefore, we hypothesize that BpOF4_01690 plays a critical role in oxidative phosphorylation under highly alkaline conditions.
在嗜碱菌OF4中注释了一种单顺反子小蛋白BpOF4_01690。它由59个氨基酸组成，具有疏水性。重要的是，仅在嗜碱菌中鉴定出该蛋白的同源物。在本研究中，在pH 10.5的低钠条件下，在基于苹果酸的限定培养基和基于葡萄糖的限定培养基中，缺失BpOF4_01690基因的突变体（命名为Δ01690）的生长比野生型弱。此外，Δ01690呼吸链的酶活性远低于野生型。这些表型与缺失突变体和缺失突变体的表型相似。因此，我们推测BpOF4_01690在高碱性条件下的氧化磷酸化中起关键作用。
