长链非编码RNA NR_026827在胃癌中的低表达
Low expression of the long non-coding RNA NR_026827 in gastric cancer.
作者信息
Zhong Fengyun, Zhu Meili, Gao Kaijian, Xu Ping, Yang Huan, Hu Duanmin, Cui Dawei, Wang Min, Xie Xiaofang, Wei Yan, Zhang Haifang, Du Hong
机构信息
Department of General Surgery, The Second Affiliated Hospital of Soochow University Suzhou 215004, Jiangsu, PR China.
Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University Suzhou 215004, Jiangsu, PR China.
出版信息
Am J Transl Res. 2018 Aug 15;10(8):2706-2711. eCollection 2018.
AIM
The contribution of long non-coding RNAs (lncRNAs) to gastric cancer associated with () infection remains largely unknown. Therefore, the present study aimed to investigate the expression of a lncRNA NR_026827 in gastric epithelial cells infected with , and demonstrate its expression characteristic in gastric cancer.
MATERIALS AND METHODS
Gastric epithelial cell line cells, GES-1, were cultured and infected with . A microarray was used to analyze the lncRNA profile of gastric epithelial cells. Eighty fresh gastric cancer tissues and the paired adjacent non-cancerous tissue samples were randomly selected from patients. The expression of the lncRNA NR_026827 was investigated using quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
The expression of several lncRNAs was significantly altered in GES-1 cells following infection with . Of these lncRNAs, NR_026827 was dramatically down-regulated in GES-1 cells infected with . In addition, the expression of NR_026827 was decreased in gastric cancer tissues in comparison to the corresponding adjacent non-cancerous tissues. Moreover, the expression of NR_026827 did not change significantly in different gastric cancer stages.
CONCLUSION
The lncRNA, NR_026827, is down-regulated in all stages of gastric cancer associated with infection and could represent a potential biomarker for the diagnosis of gastric cancer.
目的
长链非编码RNA（lncRNAs）在与（）感染相关的胃癌中的作用仍不清楚。因此，本研究旨在探讨lncRNA NR_026827在感染（）的胃上皮细胞中的表达情况，并阐明其在胃癌中的表达特征。
材料与方法
培养胃上皮细胞系GES-1细胞并感染（）。用微阵列分析胃上皮细胞的lncRNA谱。从患者中随机选取80例新鲜胃癌组织及其配对的癌旁非癌组织样本。采用定量实时聚合酶链反应（qRT-PCR）检测lncRNA NR_026827的表达。
结果
感染（）后，GES-1细胞中几种lncRNAs的表达发生显著改变。在这些lncRNAs中，NR_026827在感染（）的GES-1细胞中显著下调。此外，与相应的癌旁非癌组织相比，NR_026827在胃癌组织中的表达降低。而且，NR_026827在不同胃癌分期中的表达无明显变化。
结论
lncRNA NR_026827在与（）感染相关的胃癌各阶段均下调，可能是胃癌诊断的潜在生物标志物。
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