Kurosumi K, Koyama T, Tosaka H
Arch Histol Jpn. 1986 Jun;49(2):227-42. doi: 10.1679/aohc.49.227.
Immunoelectron microscopy using a colloidal gold-antibody method with anti-rat GH serum demonstrated three morphologically different types of GH cells in the rat anterior pituitary. They were distinguished as Types I, II and III GH cells, containing only large secretory granules about 350 nm in diameter, mixed large and small granules, and only small granules about 150 nm in diameter, respectively. Double gold labeling with large gold particles for GH and small particles for PRL or ACTH indicated that neither GH and PRL nor GH and ACTH were contained in the same cell. In adult male rats, Type I cells (68%) predominated over Type II (22%), while Type III cells were rare (9.7%). On the contrary, in the adult female rats, Type II cells (47%) slightly dominated over Type I (44%) though the rate of Type III cells was the same as in the male. In neonatal infants, the frequency of occurrence of Type III cells was as high as about 20%; sex differences between Types I and II were indistinct. The Type III cells were therefore thought to represent an immature type.
使用抗大鼠生长激素（GH）血清的胶体金-抗体法进行免疫电子显微镜检查，结果显示大鼠垂体前叶存在三种形态不同的生长激素细胞。它们分别被区分为I型、II型和III型生长激素细胞，分别只含有直径约350纳米的大分泌颗粒、大小混合颗粒以及直径约150纳米的小颗粒。用标记生长激素的大金颗粒和标记催乳素（PRL）或促肾上腺皮质激素（ACTH）的小金颗粒进行双重金标记，结果表明生长激素细胞中既不含有生长激素和催乳素，也不含有生长激素和促肾上腺皮质激素。在成年雄性大鼠中，I型细胞（68%）占主导地位，II型细胞占22%，而III型细胞很少见（9.7%）。相反，在成年雌性大鼠中，II型细胞（47%）略多于I型细胞（44%），尽管III型细胞的比例与雄性大鼠相同。在新生婴儿中，III型细胞的出现频率高达约20%；I型和II型细胞之间的性别差异不明显。因此，III型细胞被认为代表一种未成熟类型。
