Muhammad Ola I, Mahmoud Usama M, Fazio Francesco, Sayed Alaa El-Din H
Laboratory of Fish Biology and Pollution, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Department of Veterinary Sciences, Polo Universitario Annunziata, University of Messina, 98168 Messina, Italy.
Toxicol Rep. 2018 Aug 31;5:905-909. doi: 10.1016/j.toxrep.2018.08.020. eCollection 2018.
Although many studies on the hematological and biochemical parameters in fishes have been done, still there are some shortage in the estimation and evaluation of the baseline's values of marine and freshwater fishes. Recently, the use of hematology and biochemistry of fishes in toxicology, aquaculture, environmental pollution, feeding, and antioxidants studies has been increased. In this study we introduced the importance of those parameters and their importance as biomarkers in fish toxicology from previous literature and as new findings. Hemato-biochemical parameters were widely used in fish toxicological studies. Many researches have used the protein electrophoresis as a valid tool to determining intra and inter-specific variation among species. Protein profile was extensively used in determining the health of fish, as indicators of anemia or other diseases provide information about the existence of the disease, and in the diagnosis of disease. So, to carry out the aim of this study, we reported one of the more advanced techniques used SDS-PAGE as molecular biomarker for protein profile analysis in fish with shedding the light on the importance of hematological and biochemical parameters in fish toxicological studies.
尽管已经开展了许多关于鱼类血液学和生化参数的研究，但在海洋和淡水鱼类基线值的估计和评估方面仍存在一些不足。近年来，鱼类血液学和生物化学在毒理学、水产养殖、环境污染、饲料以及抗氧化剂研究中的应用有所增加。在本研究中，我们从以往文献和新发现中介绍了这些参数的重要性及其作为鱼类毒理学中生物标志物的重要性。血液生化参数在鱼类毒理学研究中被广泛应用。许多研究将蛋白质电泳作为确定物种内和种间变异的有效工具。蛋白质谱被广泛用于确定鱼类的健康状况，作为贫血或其他疾病的指标提供有关疾病存在的信息，并用于疾病诊断。因此，为了实现本研究的目的，我们报告了一种更先进的技术，即使用SDS-PAGE作为鱼类蛋白质谱分析的分子生物标志物，同时阐明血液学和生化参数在鱼类毒理学研究中的重要性。
