Tiwari Nitish Kumar, Mohanty Trupti Rani, Das Gupta Subhadeep, Roy Shreya, Swain Himanshu Sekhar, Baitha Raju, Ramteke Mitesh Hiradas, Das Basanta Kumar
National Mission For Clean Ganga Laboratory, ICAR-Central Inland Fisheries Research Institute, Barrackpore, 700120, Kolkata, India.
Environ Sci Pollut Res Int. 2023 Apr;30(16):46826-46846. doi: 10.1007/s11356-023-25519-2. Epub 2023 Feb 1.
Fishes are poikilothermic animals and are rapid responders to any sort of ecological alteration. The responses in the fish can be easily assessed from their hematological and biochemical responses. To study the variation in the hemato-biochemical parameters in retort to ecological alteration and ecological regime, a study was conducted at six different sampling stations of the middle and lower stretches of river Ganga. Various hematological and biochemical responses of fishes were also monitored in response to multiple ecological alterations. For the assessment of ecological alteration, various indices were calculated such as the water pollution index (WPI), National Sanitation Foundation-water quality index (NSF-WQI), and Nemerow's pollution index (NPI) has been calculated based on various water quality parameters such as dissolved oxygen (DO), pH, total dissolved solids (TDS), total alkalinity (TA), total hardness (TH), electrical conductivity (EC), biochemical oxygen demand (BOD), chlorinity (CL), total nitrogen (TN), and total phosphorus (TP). The hematological parameters such as WBC, RBC, platelet, hemoglobin, and hematocrit were monitored. The serum biochemical parameters such as SGPT, SGOT, ALP, amylase, bilirubin, glucose, triglyceride (TRIG), and cholesterol (CHOL) were investigated. The study revealed that NSF-WQI varied from 45.08 at Buxar to 110.63 at Rejinagar and showed a significantly positive correlation with SGPT, SGOT, ALP, TRIG, CHOL, and WBC, whereas a significantly negative correlation was observed between TRIG and RBC. WPI varied from 19 to 23 and showed a significant positive correlation with SGOT and a negative correlation was observed with total nitrogen. The PCA analysis illustrated the significance of both natural as well as anthropogenic factors on riverine ecology. Strong positive loading was observed with SGPT, SGOT, ALP, and platelet. The study signified the need for monitoring the hemato-biochemical responses of fishes in response to alterations in the ecological regime.
鱼类是变温动物，对任何形式的生态变化反应迅速。鱼类的反应可以通过其血液学和生化反应轻松评估。为了研究血液生化参数在应对生态变化和生态状况时的变化，在恒河中下游的六个不同采样站进行了一项研究。还监测了鱼类对多种生态变化的各种血液学和生化反应。为了评估生态变化，计算了各种指数，如水污染指数（WPI）、国家卫生基金会水质指数（NSF-WQI），并根据溶解氧（DO）、pH值、总溶解固体（TDS）、总碱度（TA）、总硬度（TH）、电导率（EC）、生化需氧量（BOD）、氯度（CL）、总氮（TN）和总磷（TP）等各种水质参数计算了内梅罗污染指数（NPI）。监测了白细胞、红细胞、血小板、血红蛋白和血细胞比容等血液学参数。研究了血清谷丙转氨酶（SGPT）、谷草转氨酶（SGOT）、碱性磷酸酶（ALP）、淀粉酶、胆红素、葡萄糖、甘油三酯（TRIG）和胆固醇（CHOL）等生化参数。研究表明，NSF-WQI在布克萨尔为45.08，在雷金加尔为110.63，与SGPT、SGOT、ALP、TRIG、CHOL和白细胞呈显著正相关，而TRIG与红细胞呈显著负相关。WPI在19至23之间变化，与SGOT呈显著正相关，与总氮呈负相关。主成分分析说明了自然因素和人为因素对河流生态的重要性。SGPT、SGOT、ALP和血小板呈现出较强的正负荷。该研究表明需要监测鱼类在生态状况变化时的血液生化反应。
