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Superoxide photogeneration by chlorophyll a in water/acetone. Electron spin resonance studies of radical intermediates in chlorophyll a photoreaction in vitro.

作者信息

You J L, Fong F K

出版信息

Biochem Biophys Res Commun. 1986 Sep 30;139(3):1124-9. doi: 10.1016/s0006-291x(86)80294-7.

DOI:10.1016/s0006-291x(86)80294-7
PMID:3021148
Abstract

In this paper we report the use of DMPO (5,5-dimethyl-1-pyrroline-1-oxide) as spin trap in the ESR observation of O2-. photogeneration by in vitro Ch1 a in oxygen-saturated 50:50% (v/v) water/acetone. The observed hyperfine parameters for the spin adducts of DMPO are identical to those obtained from H2O2 decomposition, and to those reported by earlier workers for the formation of 02-. in oxygen-saturated preparations of spinach chloroplasts.

摘要

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