1,5-二磷酸核酮糖羧化酶与磷酸核糖异构酶和磷酸核酮糖激酶的关联
The association of ribulose-1,5-bisphosphate carboxylase with phosphoriboisomerase and phosphoribulokinase.
作者信息
Sainis J K, Harris G C
出版信息
Biochem Biophys Res Commun. 1986 Sep 30;139(3):947-54. doi: 10.1016/s0006-291x(86)80269-8.
RuBPCase from peas showed Ribose-5-phosphate and Ribulose-5-phosphate dependent CO2 fixation when purified on sucrose gradients or by conventional methods. If purification was done in the presence of 20 mM MgCl2 and 20-25 mM NaHCO3 RuBPCase showed higher Ribose-5-phosphate and Ribulose-5-phosphate dependent CO2 fixation rates. Partially purified phosphoriboisomerase, phosphoribulokinase and RuBPCase from spinach could be reassociated in vitro.
当通过蔗糖梯度法或传统方法纯化时，豌豆中的核酮糖-1,5-二磷酸羧化酶（RuBPCase）表现出依赖于5-磷酸核糖和5-磷酸核酮糖的二氧化碳固定作用。如果在20 mM氯化镁和20 - 25 mM碳酸氢钠存在的情况下进行纯化，RuBPCase表现出更高的依赖于5-磷酸核糖和5-磷酸核酮糖的二氧化碳固定速率。菠菜中部分纯化的磷酸核糖异构酶、磷酸核酮糖激酶和RuBPCase可以在体外重新结合。
Zotero 插件