Noé G, Michotte A, Crabbé J
Biochim Biophys Acta. 1977 Aug 10;461(2):231-8. doi: 10.1016/0005-2728(77)90173-6.
The metabolic cost (in terms of oxygen consumption) of transcellular sodium transport was assessed on ventral frog skin and its isolated epithelial layers, by measuring the decrease in oxygen consumption by the tissue upon transient withdrawal of sodium from the outside solution. The same number of sodium ions was transported per molecule oxygen consumed whole skin (17.4 +/- 2.3) and its isolated epithelium (17.3 +/- 2.4). The metabolic cost of sodium transport could not be estimated properly when this process was blocked by amiloride or ouabain, as these drugs were found to bring about an increase in oxygen consumpton by the tissue when no sodium was available for transport.
通过测量从外部溶液中短暂去除钠后组织耗氧量的减少，评估了蟾蜍腹部皮肤及其分离的上皮层中跨细胞钠转运的代谢成本（以耗氧量计）。每消耗一分子氧，全皮（17.4±2.3）及其分离的上皮（17.3±2.4）转运的钠离子数量相同。当该过程被氨氯吡脒或哇巴因阻断时，无法正确估计钠转运的代谢成本，因为发现这些药物在没有钠可供转运时会导致组织耗氧量增加。
