Huang Qi, Wang Yaqiang, Xia Yao, Li Lianwei, Luo Juan, Xia Shuxian, Sun Yang, Miao Yinglei, Wang Kunhua, Chen Ye
Department of Gastroenterology, State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Gastroenterology, Guangdong Gastrointestinal Disease Research Center, Nanfang Hospital, Southern Medical University, Guangzhou Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Disease, Kunming Institute of Mathematics and Information Science, Baoji University of Arts and Sciences, Baoji, Shaanxi Computational Biology and Medical Ecology Lab, State Key Lab of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences Department of General Surgery, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of digestive disease, Kunming, China.
Medicine (Baltimore). 2018 Sep;97(37):e12248. doi: 10.1097/MD.0000000000012248.
Cystic fibrosis (CF) is a hereditary disease that is characterized by defective mucociliary clearance, airway obstruction, chronic infection, and persistent inflammation. Cystic fibrosis pulmonary exacerbation (CFPE) majorly causes the morbidity of CF patients. Although CF has been demonstrated to change the composition of lung microbial community, previous studies have not made efforts to study the differences in the mechanism of assembly and diversity maintenance of lung microbial community in CF patients. In this study, we applied the neutral theory of biodiversity to comparatively investigate the assembly and diversity maintenance of the lung microbial community before and after the antibiotic treatment by reanalyzing the dataset from Fodor et al's study. We found that no one sample in the lung microbial communities of the sputum samples of Exacerbation group, nor those of End-of-treatment group satisfied the predictions of neutral model, suggesting that the neutral-process does not dominate in CF patients before and after antibiotic treatments. By comparing the biodiversity parameter between Exacerbation and End-of-treatment group, we found that the former had the significantly higher biodiversity, but the change in diversity parameter is slight and the P value is close to.05 (P value = .41). Therefore, our second finding is that although CFPE may increase the biodiversity of lung microbial community, the change is not essential.
囊性纤维化（CF）是一种遗传性疾病，其特征为黏液纤毛清除功能缺陷、气道阻塞、慢性感染和持续性炎症。囊性纤维化肺部加重（CFPE）是导致CF患者发病的主要原因。尽管已证明CF会改变肺部微生物群落的组成，但以往研究并未着力探讨CF患者肺部微生物群落组装机制和多样性维持方面的差异。在本研究中，我们应用生物多样性中性理论，通过重新分析Fodor等人研究中的数据集，比较性地研究了抗生素治疗前后肺部微生物群落的组装和多样性维持情况。我们发现，加重期组痰液样本的肺部微生物群落以及治疗结束组痰液样本的肺部微生物群落中，均没有一个样本符合中性模型的预测，这表明在抗生素治疗前后，CF患者肺部微生物群落的中性过程并不占主导地位。通过比较加重期组和治疗结束组之间的生物多样性参数，我们发现前者的生物多样性显著更高，但多样性参数的变化很小，P值接近0.05（P值 = 0.41）。因此，我们的第二个发现是，尽管CFPE可能会增加肺部微生物群落的生物多样性，但这种变化并不显著。
