Asadi-Golshan Reza, Razban Vahid, Mirzaei Esmaeil, Rahmanian Abdolkarim, Khajeh Sahar, Mostafavi-Pour Zohreh, Dehghani Farzaneh
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
Asian Spine J. 2018 Oct;12(5):785-793. doi: 10.31616/asj.2018.12.5.785. Epub 2018 Sep 10.
Experimental animal study.
This study aimed to assess effects of conditioned medium (CM) of dental pulp-derived stem cells loaded in collagen hydrogel on functional recovery following spinal cord injury (SCI).
SCI affects sensory and motor functions, and behavioral recovery is the most essential purpose of therapeutic intervention. Recent studies have reported that CM from dental pulp-derived stem cells has therapeutic benefits. In addition, collagen hydrogel acts as a drug delivery system in SCI experiments.
Stem cells from human exfoliated deciduous teeth (SHEDs) were cultured, and SHED-CM was harvested and concentrated. Collagen hydrogel containing SHED-CM was prepared. The rats were divided into five groups receiving laminectomy, compressive SCI with or without intraspinal injection of biomaterials (SHED-CM), and collagen hydrogel with or without SHED-CM. Basso, Beattie, and Bresnahan (BBB) scoring, inclined plane, cold allodynia, and beam walk tests were performed for 6 weeks to assess locomotor, motor, sensory, and sensory-motor performances, respectively.
Scores of the rats receiving SHED-CM loaded in collagen hydrogel were significantly better than those of the other injured groups at 1-week post-injury for BBB, 2 weeks for inclined plane, 2 weeks for cold allodynia, and 4 weeks for beam walk tests (p <0.05). The differences remained significant throughout the study.
Intraspinal administration of SHED-CM loaded in collagen hydrogel leads to improved functional recovery and proposes a cell-free therapeutic approach for SCI.
实验性动物研究。
本研究旨在评估负载于胶原水凝胶中的牙髓来源干细胞条件培养基（CM）对脊髓损伤（SCI）后功能恢复的影响。
SCI会影响感觉和运动功能，行为恢复是治疗干预的最主要目的。近期研究报道，牙髓来源干细胞的CM具有治疗益处。此外，胶原水凝胶在SCI实验中可作为药物递送系统。
培养人脱落乳牙干细胞（SHEDs），收集并浓缩SHED-CM。制备含SHED-CM的胶原水凝胶。将大鼠分为五组，分别接受椎板切除术、脊髓压迫性损伤并给予或不给予脊髓内注射生物材料（SHED-CM），以及给予或不给予含SHED-CM的胶原水凝胶。在6周内分别进行Basso、Beattie和Bresnahan（BBB）评分、倾斜平面试验、冷痛觉过敏试验和横梁行走试验，以评估运动、运动、感觉和感觉运动性能。
在损伤后1周的BBB评分、2周的倾斜平面试验、2周的冷痛觉过敏试验以及4周的横梁行走试验中，接受负载于胶原水凝胶中的SHED-CM的大鼠得分显著高于其他损伤组（p<0.05）。在整个研究过程中，差异均保持显著。
脊髓内给予负载于胶原水凝胶中的SHED-CM可改善功能恢复，并为SCI提出了一种无细胞治疗方法。
